Transcriptomics Combined with Photosynthetic Physiology and Leaf Structure Analysis Revealed Increased Sugarcane Yield by Fenlong-Ridging

Abstract

Fenlong-ridging (FL) is a new type of conservation tillage. In many crops, FL increases crop yield and quality; however, the cytology and molecular mechanisms of crops under FL is not completely understood. This study investigated soil physical and chemical properties under FL and conventional tillage (CK) during 2018–2019 (plant cane) and 2019–2022 (first stubble), and analyzed the agronomic trait, physiology, leaf anatomical structure, and gene expression related to photosynthesis between FL and CK of sugarcane (Guitang 42). Soil bulk density significantly increased, and soil porosity, water storage, and content of available nitrogen and phosphorus under FL were significantly higher than those under CK. Plant height, stem diameter, single stem weight, effective stem number and yield significantly increased under FL compared to under CK. Sugar content significantly increased in plant cane under FL. Chlorophyll content and the photosynthetic rate increased, with significantly higher activity of photosynthetic enzymes including NADP-malate dehydrogenase (NADP-MDH), phosphoenolpyruvate carboxylase (PEPC), and ribulose-1,5-bisphosphate carboxylase (RuBPC) under FL compared to CK. Fenlong-ridging cytology results showed that the mesophyll cells were large and arranged well, the Kranz anatomy was noticeable, and there were a high number of large chloroplasts in mesophyll cell and in the vascular bundle sheath. Furthermore, the bundle sheath in FL was larger than that in CK. Transcriptomics results showed that 19,357 differentially genes (DEGs) were up-regulated and 28,349 DEGs were down-regulated in sugarcane leaves under FL vs. CK. The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that abundant DEGs were enriched in photosynthesis, photosynthesis-antenna protein, carotenoid biosynthesis, and other pathways associated with photosynthesis. Most expression was up-regulated, thus, facilitating photosynthesis regulation. Quantitative real-time polymerase chain reaction analysis revealed the up-regulation of genes related to photosynthesis (PsaH and PsbS) under FL. Overall, this study provides insights into the role of FL in increased sugarcane yield by integrating physiology, cytology, and proteomics analysis. These findings could be used to further improve its application and promotion.