Abstract
Background: Cancer is one of the major human problems and breast cancer is a significant health problem. In spite of the developed treatment methods, breast cancer cannot be treated very effectively. Therefore, the identification of novel genes that will have played a role in the treatment and diagnosis of breast cancer and the high interest of B-cell lymphoma (BCL2) family proteins are critical regulators of apoptosis). Objective: The current study aimed to investigate the relationship between the levels of BCL2, BAX, and B-cell integrate medium (BIM) proteins which the prognosis and apoptosis processes of the breast cell line. Patients and Methods: Collected tissue samples from normal and cancer tissue type, RNA isolated from the cell, then quantitation done, cDNA synthesized, cDNA quantitation and Real-time PCR was performed to see the level between two kinds of genes. Results: BCL2 protein level was significantly lessened in regulator molecule tissues of a cell line with breast cancer. In contrast to Bax protein, no significant difference was detected in the BIM expression level. Our result also showed that low BCL2 expression level is associated with significant clinical characteristics of the patient such as tumor grade, stage and breast cancer type. Conclusion: Findings of our study showed that BCL2 has a role in breast cancer formation and might be a novel biomarker for the diagnosis and treatment of breast cancer.
Highlights
Breast cancer is a major health problem in all countries and predominantly affects women population.1It is the most common type of cancer in women and responsible for the most cancer-related death among women population[2].In the United States, it is estimated that 255,180 new cases of breast cancer and 41,070 breast cancer-related deaths will occur3
In Table (1) B-cell CLL/lymphoma 2 (BCL2) expression levels of breast cancer patient were shown according to age (65)
BCL2 protein levels compared with the age distribution, it was found to be more prevalent in 65 year age tumor samples
Summary
Breast cancer is a major health problem in all countries and predominantly affects women population.1It is the most common type of cancer in women and responsible for the most cancer-related death among women population[2].In the United States, it is estimated that 255,180 new cases of breast cancer and 41,070 breast cancer-related deaths will occur. In many types of cancer, tumor cells gain resistance to apoptotic death by inducing the expression of anti-apoptotic Bcl-2 proteins and inhibiting the expression of pro-apoptotic proteins such as Bax, leading to disruption of the p53 mechanism. The identification of novel genes that will have played a role in the treatment and diagnosis of breast cancer and the high interest of B-cell lymphoma (BCL2) family proteins are critical regulators of apoptosis). Objective: The current study aimed to investigate the relationship between the levels of BCL2, BAX, and B-cell integrate medium (BIM) proteins which the prognosis and apoptosis processes of the breast cell line. Results: BCL2 protein level was significantly lessened in regulator molecule tissues of a cell line with breast cancer. Our result showed that low BCL2 expression level is associated with significant clinical characteristics of the patient such as tumor grade, stage and breast cancer type.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
