Abstract
Parkinson’s disease (PD) is the second most common neurodegenerative disease. However, the expression pattern and the differential gene in different brain regions and its functions remain unclear although many studies have been reported. In this present study, PD mouse model were build and four brain regions (cerebral cortex: CC, hippocampus: HP, striatum: ST, and cerebellum: CB) were separated for RNA-seq analysis. Results showed that different expressed genes were found between the different brain regions and more differential genes found in ST and HP when compared with control groups. Among them, Lrrk2, Mtor, Gxylt1, C920006o11Rik, Vdac1, Drd4, and Ncan showed the most significant to PD. PDCC vs. PDHP, PDHP vs. PDST and PDCC vs. PDST groups have 334, 722 and 495 differentially expressed genes (DEGs), respectively. Functional analyses results showed that the differential genes mainly related with posttranscriptional regulation of gene expression and protein localization to organelle and so on, which involved in AMPK, PI3K-Akt signaling pathway, and GABA-ergic synapse. Network biology analysis showed LRRK2, DRD2, IGF-1, GNAI1, GNAI3, PRKACA, PPP2R5C, and PIK3R1 play a major role in protein regulation of PD. Therefore, HP and ST play more important roles in the development of PD and it is also suggested the potential target gene for diagnosis and treatment of PD.
Highlights
MethodsC57Bl/6J mice (8 weeks old) were included in the present study
Parkinson’s disease (PD) is the second most common neurodegenerative disease and many studies have researched its complex pathophysiological processes
The results showed that leucine-rich repeat kinase 2 (LRRK2), DA receptor D2 (DRD2), insulin-like growth factor 1 (IGF-1), guanine nucleotide binding protein alpha inhibiting 1 (GNAI1), guanine nucleotide binding protein alpha inhibiting 3 (GNAI3), protein kinase A catalytic subunits (PRKACA), PPP2R5C, and PIK3R1 played a major role in protein regulation
Summary
C57Bl/6J mice (8 weeks old) were included in the present study. Eight male mice were randomly divided into a control group and a model group, with four mice in each group. After 3 days of acclimation, the mice in the model group were tested on pole test and rotarod test to collect baseline data. The mice in the model group were intraperitoneally injected with MPTP (Sigma, St. Louis, MO, USA) solution every day at a dose of 20 mg/kg for 10 days. The control group mice were injected with normal saline. After 10 days, all the mice in the model group were tested for pole test and rotarod test, and the mice with obvious impairment in their sports ability were screened for subsequent experiments. The study was reviewed and approved by the Ethics Committee of Zhongda Hospital Southeast University
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