Abstract
Myo-insositol (MI) is a crucial substance in the growth and developmental processes in plants. It is commonly added to the culture medium to promote adventitious shoot development. In our previous work, MI was found in influencing Agrobacterium-mediated transformation. In this report, a high-throughput RNA sequencing technique (RNA-Seq) was used to investigate differently expressed genes in one-month-old Arabidopsis seedling grown on MI free or MI supplemented culture medium. The results showed that 21,288 and 21,299 genes were detected with and without MI treatment, respectively. The detected genes included 184 new genes that were not annotated in the Arabidopsis thaliana reference genome. Additionally, 183 differentially expressed genes were identified (DEGs, FDR ≤0.05, log2 FC≥1), including 93 up-regulated genes and 90 down-regulated genes. The DEGs were involved in multiple pathways, such as cell wall biosynthesis, biotic and abiotic stress response, chromosome modification, and substrate transportation. Some significantly differently expressed genes provided us with valuable information for exploring the functions of exogenous MI. RNA-Seq results showed that exogenous MI could alter gene expression and signaling transduction in plant cells. These results provided a systematic understanding of the functions of exogenous MI in detail and provided a foundation for future studies.
Highlights
Myo-inositol (MI) is a small molecule that is important in many different developmental and physiological processes in eukaryotic cells [1, 2]
The results showed that tumorigenesis efficiency in the roots grown with the addition of exogenous MI was significantly higher than the control with no MI addition except 1000 mg l-1 MI concentration (S1B and S1D Fig)
The differentially expressed genes (DEGs) are involved in subcategories of biological process (BP) and molecular function (MF) (Fig 3B and 3C)
Summary
Myo-inositol (MI) is a small molecule that is important in many different developmental and physiological processes in eukaryotic cells [1, 2]. MI participates in the phosphatidylinositol (PtdIns) signaling pathway, auxin storage and transport, phytic acid biosynthesis, cell wall biosynthesis, and the production of stress-related molecules [3, 4]. MI is commonly added in plant culture media, as its addition is believed to improve plant regeneration [5,6,7]. Because of PLOS ONE | DOI:10.1371/journal.pone.0161949 September 7, 2016
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