Abstract
Opium poppy is one of the most important medicinal plants and remains the only commercial resource of morphinan-based painkillers. However, little is known about the regulatory mechanisms involved in benzylisoquinoline alkaloids (BIAs) biosynthesis in opium poppy. Herein, the full-length transcriptome dataset of opium poppy was constructed for the first time in accompanied with the 33 samples of Illumina transcriptome data from different tissues, growth phases and cultivars. The long-read sequencing produced 902,140 raw reads with 55,114 high-quality transcripts, and short-read sequencing produced 1,923,679,864 clean reads with an average Q30 rate of 93%. The high-quality transcripts were subsequently quantified using the short reads, and the expression of each unigene among different samples was calculated as reads per kilobase per million mapped reads (RPKM). These data provide a foundation for opium poppy transcriptomic analysis, which may aid in capturing splice variants and some non-coding RNAs involved in the regulation of BIAs biosynthesis. It can also be used for genome assembly and annotation which will favor in new transcript identification.
Highlights
Background & SummaryOpium poppy (Papaver somniferum) is one of the most important medicinal plants in the world and remains the only commercial resource of morphinan-based painkillers[1]
Investigation into the benzylisoquinoline alkaloids (BIAs) biosynthesis mechanism began in the 1960s with radiotracer technology[3]
Some key steps in the BIA biosynthesis pathway are yet to be identified, and there has been a lack of investigation into the molecular mechanisms of gene regulation in the pathway[3]
Summary
Opium poppy (Papaver somniferum) is one of the most important medicinal plants in the world and remains the only commercial resource of morphinan-based painkillers[1]. Little research has been conducted on the regulatory mechanisms in opium poppy despite the recently release of its genome[1] The reason for this oversight may be the lack of the transcriptional information on opium poppy in different growth periods/status or tissues. To date, there has been no report on a full-length transcriptome dataset of opium poppy, despite the recent release of its genome[1]. We construct the full-length transcriptome dataset of opium poppy using twenty-one pooled RNA samples from three different tissues and five different growth phases (Fig. 1). This produced 902,140 post-filter polymerase reads and 660,418 circular consensus sequence (CCS) reads (Table 1 and Fig. 2). It can be used to analyze the regulation mechanisms of BIAs biosynthesis according to its tissue-specific distribution, dynamic accumulation in different growth phases as well as BIAs diversity in different germplasm resources
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