Transcriptomic evidence of adaptive tolerance to high environmental ammonia in mudskippers

Abstract

Mudskippers are typical amphibious fishes and possess various strategies to ameliorate ammonia toxicity during exposure to environmental ammonia. The present study aimed to provide transcriptomic evidence through profiling the gill and liver transcriptomes of Boleophthalmus pectinirostris (BP) and Periophthalmus magnuspinnatus (PM), which were subjected to treatment with high environmental ammonia for up to 72 h. The results of gene function annotation showed that most of the differentially expressed genes were involved in metabolic pathways. After ammonia exposure, the protein and amino acid metabolism related genes in mudskippers were down-regulated, and PM had more down-regulated genes than BP. The expression levels of several representative genes involved in ammonia excretion in the gill were commonly increased. Interestingly, NH4+ transporting and H+ excreting related genes, including Na+/K+(NH4+)/2Cl− cotransporter (nkcc), Na+/K+(NH4+)-ATPase (nka), carbonic anhydrase 2 (ca2), H+-ATPase, Na+/H+ (NH4+)-exchanger (nhe), and carbonic anhydrase 15 (ca15), were up-regulated more significantly in BP than PM; however, the transcription levels of Rhesus glucoprotein b (Rhbg) and Rhesus glucoprotein c1 (Rhcg1), which constitute the NH3 transporting channels, were up-regulated more significantly in PM than BP. Furthermore, the present study provides molecular evidence for how mudskippers adopt partial amino acid catabolism to decrease the production of endogenous ammonia under high environmental ammonia loading.