Transcriptomic data of mature oil palm basal trunk tissue infected with Ganoderma boninense

Nur Qistina Othman,Suhaila Sulaiman,Yang Ping Lee,Joon Sheong Tan

doi:10.1016/j.dib.2019.104288

Abstract

To date, Ganoderma boninense is known to be the causal agent of basal stem rot (BSR) disease in oil palm (Elaeis guineensis). This disease causes rotting in the roots, basal and upper stem of oil palm. Infection causes progressive destruction of the basal tissues at the oil palm trunk and internal dry rotting, particularly at the intersection between the bole and trunk. Molecular responses of oil palm during infection are not well study although this information is crucial to strategize effective measures to control or eliminate BSR. Here we report three sets of transcriptome data from samples of near-rot section of basal stem tissue of oil palm tree infected with G. boninense (IPIT), healthy section of basal stem tissue of the same G. boninense infected palm (IPHT) and the healthy section of basal stem tissue of the healthy palm (HPHT). The raw reads were deposited into NCBI database and can be accessed via BioProject accession number PRJNA530030.

Highlights

Ganoderma boninense is known to be the causal agent of basal stem rot (BSR) disease in oil palm (Elaeis guineensis)
We report three sets of transcriptome data from samples of near-rot section of basal stem tissue of oil palm tree infected with G. boninense (IPIT), healthy section of basal stem tissue of the same G. boninense infected palm (IPHT) and the healthy section of basal stem tissue of the healthy palm (HPHT)
A pair of healthy and infected clonal mature palm was selected from Serting Hilir field research station, Negeri Sembilan, Malaysia owned by Felda Agricultural Services Sdn Bhd

Summary

Sampling materials

A pair of healthy and infected clonal mature palm was selected from Serting Hilir field research station, Negeri Sembilan, Malaysia owned by Felda Agricultural Services Sdn Bhd. Briefly, the bark was marked according to the health status. The healthy section (IPHT) and near-rot section (IPIT) of infected oil palm were collected from the cross section of the basal stem. The cross section of healthy tissue section of healthy oil palm tree (HPHT) was collected. The healthy palm was determined by healthy oil palm phenotype with no G. boninense fruiting body attached at the basal stem and the palm does not exhibit stress-related symptoms phenotypically. All collected basal stem tissues were immediately submerged in liquid nitrogen and kept at À80 C freezer until further processing for RNA extraction

RNA extraction and quality assessment of total RNA

Transcriptome sequencing

Pre-processing of RNA-seq raw data and transcriptome mapping