Abstract
Resveratrol is a natural polyphenol compound produced in response to biotic and abiotic stresses in grape berries. However, changes in resveratrol caused by bud sport in grapes are scarcely reported. In this study, trans-resveratrol and cis-resveratrol were identified and quantified in the grape berries of ‘Summer Black’ and its bud sport ‘Nantaihutezao’ from the veraison to ripening stages using ultra performance liquid chromatography-high resolution tandem mass spectrometry (UPLC-HRMS). We found that bud sport accumulates the trans-resveratrol earlier and increases the contents of cis-resveratrol in the earlier stages but decreases its contents in the later stages. Simultaneously, we used RNA-Seq to identify 51 transcripts involved in the stilbene pathways. In particular, we further identified 124 and 19 transcripts that negatively correlated with the contents of trans-resveratrol and cis-resveratrol, respectively, and four transcripts encoding F3'5'H that positively correlated with the contents of trans-resveratrol by weighted gene co-expression network analysis (WGCNA). These transcripts may play important roles in relation to the synergistic regulation of metabolisms of resveratrol. The results of this study can provide a theoretical basis for the genetic improvement of grapes.
Highlights
Resveratrol (C14H12O3; 3,4',5-trihydroxystilbene) is a beneficial secondary metabolite that belongs to the stilbene family, consisting of two aromatic rings joined by a methylene bridge, and exists in two isomers, trans-resveratrol and cis-resveratrol (Stervbo et al, 2007; Guerrero et al, 2010; Figure 1)
Transcriptome analysis provides an insight into the phenylalanine ammonialyase (PAL), 4-coumarate: coenzyme A ligase (4CL), and STS expression profiles showing that the concentration of their transcripts increased from veraison to maturity, as well as the content of resveratrol increases in the grape (Gatto et al, 2008)
We previously found that the resveratrol content and its composition in berry peel were different between the two cultivars, which might constitute a useful experimental system for the study of resveratrol metabolic mechanism and will help us to better understand the mechanism of grape ripening underlying the bud sport variety
Summary
Resveratrol (C14H12O3; 3,4',5-trihydroxystilbene) is a beneficial secondary metabolite that belongs to the stilbene family, consisting of two aromatic rings joined by a methylene bridge, and exists in two isomers, trans-resveratrol and cis-resveratrol (Stervbo et al, 2007; Guerrero et al, 2010; Figure 1). The difference between the chemical structures of the cis-resveratrol and trans-resveratrol is the geometry of carbon–carbon double bond (Huang and Mazza, 2011). Resveratrol is derived from the phenylpropanoid pathway that involves a series of enzymes such as phenylalanine ammonialyase (PAL), 4-coumarate: coenzyme A ligase (4CL), cinnamic acid 4-hydroxylase (C4H), and stilbene synthase (STS; Gatto et al, 2008; Wang et al, 2010; Duan et al, 2015). Trans-resveratrol is a leading and important stilbene compound, but cis-resveratrol has not been detected often (Liu et al, 2010). Resveratrol can be transported to different tissues of grapevine subjected to stress and adaptation mechanisms in response to the environment, which may cause resveratrol to be converted to its isomers (Ji et al, 2014). The competition between STS and chalcone synthase (CHS) for the same substrates is the factor that affects the accumulation of resveratrol (Jeandet et al, 1995; Gatto et al, 2008; Yin et al, 2016; Lu et al, 2021)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
