Abstract
BackgroundCotton is an important fiber crop but has serious heterosis effects, and cytoplasmic male sterility (CMS) is the major cause of heterosis in plants. However, to the best of our knowledge,no studies have investigated CMS Yamian A in cotton with the genetic background of Australian wild Gossypium bickii. Conjoint transcriptomic and proteomic analysis was first performed between Yamian A and its maintainer Yamian B.ResultsWe detected 550 differentially expressed transcript-derived fragments (TDFs) and at least 1013 proteins in anthers at various developmental stages. Forty-two TDFs and 11 differentially expressed proteins (DEPs) were annotated by analysis in the genomic databases of G. austral, G. arboreum and G. hirsutum. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed to better understand the functions of these TDFs and DEPs. Transcriptomic and proteomic results showed that UDP-glucuronosyl/UDP-glucosyltransferase, 60S ribosomal protein L13a-4-like, and glutathione S-transferase were upregulated; while heat shock protein Hsp20, ATPase, F0 complex, and subunit D were downregulated at the microspore abortion stage of Yamian A. In addition, several TDFs from the transcriptome and several DEPs from the proteome were detected and confirmed by quantitative real-time PCR as being expressed in the buds of seven different periods of development. We established the databases of differentially expressed genes and proteins between Yamian A and its maintainer Yamian B in the anthers at various developmental stages and constructed an interaction network based on the databases for a comprehensive understanding of the mechanism underlying CMS with a wild cotton genetic background.ConclusionWe first analyzed the molecular mechanism of CMS Yamian A from the perspective of omics, thereby providing an experimental basis and theoretical foundation for future research attempting to analyze the abortion mechanism of new CMS with a wild Gossypium bickii background and to realize three-line matching.
Highlights
Cotton is an important fiber crop but has serious heterosis effects, and cytoplasmic male sterility (CMS) is the major cause of heterosis in plants
Production practice shows that CMS is an effective method for heterosis utilization in crops and is widely used to produce hybrid seeds because it eliminates the need for artificial emasculation, saves manpower and material resources, enhances the purity of hybrid seeds and increases the output of crops [3, 4]
To the best of our knowledge, there is no report on CMS in cotton with the genetic background of Australian wild Gossypium bickii, which has been reported despite the considerable effects of heterosis in cotton germplasm development
Summary
Cotton is an important fiber crop but has serious heterosis effects, and cytoplasmic male sterility (CMS) is the major cause of heterosis in plants. To the best of our knowledge, no studies have investigated CMS Yamian A in cotton with the genetic background of Australian wild Gossypium bickii. The adoption of the production of hybrid seeds is the most important among various means of utilizing cotton heterosis. Castration in the production of hybrid seeds often relies on hand emasculation and male-sterile lines produced by such means as chemically induced male sterility, genic male sterility, and cytoplasmic male sterility (CMS) [3]. To the best of our knowledge, there is no report on CMS in cotton with the genetic background of Australian wild Gossypium bickii, which has been reported despite the considerable effects of heterosis in cotton germplasm development
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