Abstract
BackgroundRose is one of the most popular flowers in the wold. Its field growth and quality are negatively affected by aphids. However, the defence mechanisms used by rose plants against aphids are unclear. Therefore, to understand the defence mechanism of rose under aphid stress, transcriptome and metabolome techniques were used to investigate the regulation mechanism in R. longicuspis infected with M. rosivorum.ResultIn our study, after inoculation with M. rosivorum, M. rosivorum quickly colonized R. longicuspis. A total of 34,202 genes and 758 metabolites were detected in all samples. Under M. rosivorum stress, R. longicuspis responded by MAPK cascades, plant hormone signal transduction pathway activation, RlMYBs and RlERFs transcription factors expression and ROS production. Interestingly, the ‘brassinosteroid biosynthesis’ pathway was significantly enriched in A3 d-vs.-A5 d. Further analysis showed that M. rosivorum induced the biosynthesis of secondary metabolites such as terpenoids, tannins and phenolic acids, among others. Importantly, the ‘glutathione metabolic’ and ‘glucosinolate biosynthesis’ pathways were significantly enriched, which involved in the rose against aphids.ConclusionOur study provides candidate genes and metabolites for Rosa defence against aphids. This study provides a theoretical basis for further exploring the molecular regulation mechanism of rose aphid resistance and aphid resistance breeding in the future.
Highlights
Rose is one of the most popular flowers in the wold
This study provides a theoretical basis for further exploring the molecular regulation mechanism of rose aphid resistance and aphid resistance breeding in the future
Aphid population statistics and leaf morphological changes after inoculation with M. rosivorum on R. longicuspis To understand the reproduction of aphids, we recorded the number of aphids at 0 d, 1 d, 3 d, 5 d and 7 d
Summary
Rose is one of the most popular flowers in the wold. Its field growth and quality are negatively affected by aphids. Aphids are the most common predator and affect the yield, quality and ornamental value of rose [2]. Aphids often gather in the immature tissues of roses and damage the immature leaves, shoots, branches and buds of roses by sucking the juice, causing leaf curling, yellowing or abnormal flowering [3]. These aphid species include Macrosiphum rosae, Macrosiphum rosivorum, Myzus persicae, Myzaphis rosarum and Aphis gossypii. Macrosiphum rosivorum is the most common and serious in rose [4, 5] At present, insecticides such as pymetrozine and imidacloprid are mainly used to control aphids on rose crops. The worse the effect, the larger the drug dosage, which increases the
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