Transcriptomic and evolutionary analyses of white pear (Pyrus bretschneideri) β-amylase genes reveals their importance for cold and drought stress responses

Abstract

β-amylase (BAM) genes play essential roles in plant abiotic stress responses. Although the genome of Chinese white pear (Pyrus bretschneideri) has recently been made available, knowledge regarding the BAM family in pear, including gene function, evolutionary history and patterns of gene expression remains limited. In this study, we identified 17 PbBAMs in the pear genome. Of these, 12 PbBAM members were mapped onto 9 chromosomes and 5 PbBAM genes were located on scaffold contigs. Based on gene structure, protein motif analysis, and the topology of the phylogenetic tree of the PbBAM family, we classified member genes into 4 groups. All PbBAM genes were found to contain typical glycosyl hydrolysis 14 domain motifs. Interfamilial comparisons revealed that the phylogenetic relationships of BAM genes in other Rosaceae species were similar those found in pear. We also found that whole-genome duplication (WGD)/segmental duplication events played critical roles in the expansion of the BAM family. Next, we used transcriptomic data to study gene expression during the response of drought and low temperate responses, and found that genes in Group B were related to drought and cold stress. We identified four PbBAM genes associated with abiotic stress in Pear. Finally, by analyzing co-expression networks and co-regulatory genes, we found that PbBAM1a and PbBAM1b were associated with the pear abiotic stress response.