Transcriptomic Analysis Reveals Cu/Zn SODs Acting as Hub Genes of SODs in Hylocereus undatus Induced by Trypsin during Storage.

Xinyue Pang,Luning Cai,Xin Li,Bairu Li,Xueru Liu,Xinling Li

doi:10.3390/antiox9020162

Xinyue Pang, Luning Cai + Show 4 more

Open Access

https://doi.org/10.3390/antiox9020162

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Journal: Antioxidants	Publication Date: Feb 17, 2020
Citations: 8	License type: CC BY 4.0

Affiliation: Henan University of Science and Technology

Abstract

It has been revealed by us that superoxide scavenging is a new activity of trypsin. In this study, the synergistic mechanisms of trypsin and superoxide dismutases (SODs) were evaluated in Hylocereus undatus (pitaya). Trypsin significantly improved the storage quality of H. undatus, including weight loss impediment and decrease of cellular injury. The regulatory mechanisms of 16 SOD genes by trypsin were revealed using transcriptomic analysis on H. undatus. Results revealed that important physiological metabolisms, such as antioxidant activities or metal ion transport were induced, and defense responses were inhibited by trypsin. Furthermore, the results of protein–protein interaction (PPI) networks showed that besides the entire ROS network, the tiny SODs sub-network was also a scale-free network. Cu/Zn SODs acted as the hub that SODs synergized with trypsin during the storage of H. undatus.

Highlights

Hylocereus undatus (H. undatus), is a species of Cactaceae [1]
This study explored several questions abouteither the effect of trypsin treatment on SODthe accumulation of reactive oxygen species (ROS)
The trypsin treatment significantly reduced the accumulation of endogenous ROS, including O2 −

Summary

Introduction

Based on the commercial value and outstanding nutritional properties, H. undatus is becoming increasingly important to the world [2]. The quality of H. undatus decreases with time during storage. Few works have aimed to increase the storage quality of H. undatus [3]. The disequilibrium of reactive oxygen species (ROS) production and scavenging will lead to elevated ROS levels, and further induce an increase of cellular injury and result in fruit spoilage by the end of storage of fruit [4,5]. The activities of superoxide dismutase (SOD) have been reported to be induced during fruit spoilage [7], while the regulatory mechanisms among three groups (iron SOD (FSD), copper/zinc SOD (CSD), and manganese SOD (MSD)) during fruit storage have not been elucidated to date

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