Abstract
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a destructive disease of wheat worldwide. The disease is preferably controlled by growing resistant cultivars. Wheat cultivar Xiaoyan 6 (XY 6) has been resistant to stripe rust since its release. In the previous studies, XY 6 was found to have higher-temperature seedling-plant (HTSP) resistance. However, the molecular mechanisms of HTSP resistance were not clear. To identify differentially expressed genes (DEGs) involved in HTSP resistance, we sequenced 30 cDNA libraries constructed from XY 6 seedlings exposed to several temperature treatments. Compared to the constant normal (15°C) and higher (20°C) temperature treatments, 1395 DEGs were identified in seedlings exposed to 20°C for 24 h (to activate HTSP resistance) and then kept at 15°C. These DEGs were located on all 21 chromosomes, with 29.2% on A, 41.1% on B and 29.7% on D genomes, by mapping to the Chinese Spring wheat genome. The 1395 DEGs were enriched in ribosome, plant-pathogen interaction and glycerolipid metabolism pathways, and some of them were identified as hub proteins (phosphatase 2C10), resistance protein homologs, WRKY transcription factors and protein kinases. The majority of these genes were up-regulated in HTSP resistance. Based on the differential expression, we found that phosphatase 2C10 and LRR receptor-like serine/threonine protein kinases are particularly interesting as they may be important for HTSP resistance through interacting with different resistance proteins, leading to a hypersensitive response.
Highlights
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a destructive disease affecting wheat production world wide (Wan, 2003; Chen, 2005)
High-temperature adult plant (HTAP) resistance to Pst becomes effective when wheat plants were grown under a night/day cycle of 10∼12◦C/25∼30◦C after inoculation (Chen, 2005, 2013; Coram et al, 2008a; Bryant et al, 2014)
Wheat crops grown in the fields are almost all under fluctuating temperature conditions every day and changing temperatures throughout the growth season, and screening germplasm for stripe rust resistance should be carried out at diurnally changed temperatures (Chen, 2013)
Summary
Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a destructive disease affecting wheat production world wide (Wan, 2003; Chen, 2005). Breeding resistant cultivars is the best approach for controlling stripe rust (Zhang et al, 2001; Chen, 2007; Sui et al, 2009). Different types of stripe rust resistance have been identified and used for developing resistant cultivars. Race-specific resistance is usually controlled by major genes and effective throughout plant development. New virulent Pst races can overcome race-specific resistance (Chen, 2005; Zheng et al, 2013). The rapid development of Pst races that have overcome Yr2, Yr9, Yr17, and Yr27 resistance has led to destructive epidemics in many parts of the world (Wellings, 2011). Non-race-specific resistance is usually quantitative and often controlled by several genes (Coram et al, 2008a; Chen, 2013)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
