Abstract
Taodan granules (TDGs) are clinically efficacious for treating psoriasis, buttheir specific mechanisms of action are unclear. In this study, we determined the concentrations of tanshinone IIA and curcumol using high-performance liquid chromatography (HPLC) to establish quality control parameters for assessing the mechanism of TDGs in treating psoriasis. Thereafter, a mouse model of psoriasis was treated with TDGs. TDGs attenuated imiquimod-induced typical erythema, scales, and thickening of the back and ear lesions in the psoriatic mouse model. Furthermore, PCNA and Ki67-positive cells were reduced in the epidermis of psoriatic lesions following TDG treatment. Finally, the sequencing results were verified using a multitude of methods, and the mechanism of action of TDGs against psoriasis was found to be via the upregulation of metabolic signaling pathways such as the Gly-Ser-Thr axis, the downregulation of immune and inflammatory pathways, and the decrease in Rac2 and Arhgdib concentrations. Overall, this study clarified the mechanism of TDG treatment for psoriasis and provided evidence for its clinical application.
Highlights
Psoriasis is a common chronic refractory skin disease, characterized by epidermal hyperkeratosis, angiogenesis, and inflammatory reactions (Armstrong et al, 2020)
Expression Analysis We investigated the difference in gene expression with Taodan granules (TDGs) treatment, RNA was extracted from the back tissues of the IMQ and IMQ + TDG groups for mRNA sequencing on day 12
We affirmed the protein expression of Arhgdib in the back lesions through western blotting, consistent with the trend of expression by ELISA (Figure 5C). These results suggest that TDGs may alleviate the symptoms of psoriasis by regulating related C3 botulinum substrate 2 (Rac2) and Arhgdib, which are related to the mechanism of cell migration
Summary
Psoriasis is a common chronic refractory skin disease, characterized by epidermal hyperkeratosis, angiogenesis, and inflammatory reactions (Armstrong et al, 2020). The disease is associated with a significant economic burden, while the all-cause healthcare cost of each patient with psoriasis per year is generally $12,523 (Al Sawah et al, 2017). Psoriasis is a systemic disease that involves the skin, but is associated with comorbidities, including cardiovascular disease, chronic obstructive. Systemic inflammation and oxidative stress in psoriasis are closely related to a variety of complications associated with a conspicuously high risk of death in patients with conditions such as kidney disease and liver disease (Dhana et al, 2019). Chinese medicine (CM) has become a global alternative medicine that has been gradually adopted for the treatment of psoriasis (Parker et al, 2017)
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