Transcriptomic Analysis of Fuzi Lizhong Decoction for the Treatment of Stomach Ulcers.

Yang Xin,Haijun Wang,Lei Xu

doi:10.1155/2020/3291853

Yang Xin, Haijun Wang + Show 1 more

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https://doi.org/10.1155/2020/3291853

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Abstract

This study aims to understand the treatment of stomach ulcers with FLD and to identify its potential target genes as well as related pathways by transcriptomic analysis. Rat stomach mRNA from a blank group (BG), a model group (MG), an untreated-model group (u-MG), and a treated group (TG) was sequenced. A partial least-squares discriminant analysis (PLS-DA) was used to differentiate the MG from the BG, and the Deseq2 R Package was used to identify differentially expressed genes between these groups. Furthermore, t-tests based on transcripts per million (TPM) were used to select different genes between MG and BG and significantly retrieved genes in TG, except for self-retrieved genes in u-MG. Finally, pathways regulated by retrieved genes were analyzed with KEGG database. Results showed that 327 of the 32,623 total detected genes were different (p < 0.05) between the MG and BG. Among these genes, eighteen genes were significantly retrieved after rats were treated with FLD in TG, and they were considered as target genes on which FLD acted. In conclusion, FLD was deduced to cure stomach ulcers by affecting glycerolipid metabolism, fatty acid degradation, circadian entrainment, circadian rhythm, and dopaminergic synapse.

Highlights

Stomach ulcers are common digestive diseases, and they may develop into serious diseases, such as stomach cancer, if not treated in time
Because the reads count can be affected by gene length and sequencing depth, their absolute quantity may not be proportional to the real expression level. erefore, the transcripts per million (TPM) was calculated by StringTie (1.3.3b) software to correct the above effect and evaluate the gene expression level. ere were a total of 32,623 genes with calculated expression profiles
The curative effect and mechanism of Fuzi Lizhong Decoction (FLD) on stomach ulcers were assessed by tissue transcriptome analysis coupled with PCR technology

Summary

Introduction

Stomach ulcers are common digestive diseases, and they may develop into serious diseases, such as stomach cancer, if not treated in time. Stomach ulcer causes the body to be in a condition of poor health. Erefore, it is important to prevent and treat stomach ulcers to maintain health. Erefore, studies on stomach ulcers are of interest to scholars. An increasing number of studies have been committed to explore stomach ulcer pathogenesis [2, 3] and to identify effective pharmaceuticals to treat stomach ulcers [4, 5]. In China, FLD is primarily used to treat digestive disease including chronic gastritis [6], chronic ulcerative colitis [7], and functional dyspepsia [8]. E FLD pill was included in the Chinese Pharmacopoeia (2015 version) In China, FLD is primarily used to treat digestive disease including chronic gastritis [6], chronic ulcerative colitis [7], and functional dyspepsia [8]. e FLD pill was included in the Chinese Pharmacopoeia (2015 version)

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Results

Conclusion