Abstract
Flower blooming is a critical and complicated plant developmental process in flowering plants. However, insufficient information is available about the complex network that regulates flower blooming in Jasminum sambac. In this study, we used the RNA-Seq platform to analyze the molecular regulation of flower blooming in J. sambac by comparing the transcript profiles at two flower developmental stages: budding and blooming. A total of 4577 differentially-expressed genes (DEGs) were identified between the two floral stages. The Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses revealed that the DEGs in the “oxidation-reduction process”, “extracellular region”, “steroid biosynthesis”, “glycosphingolipid biosynthesis”, “plant hormone signal transduction” and “pentose and glucuronate interconversions” might be associated with flower development. A total of 103 and 92 unigenes exhibited sequence similarities to the known flower development and floral scent genes from other plants. Among these unigenes, five flower development and 19 floral scent unigenes exhibited at least four-fold differences in expression between the two stages. Our results provide abundant genetic resources for studying the flower blooming mechanisms and molecular breeding of J. sambac.
Highlights
ObjectivesThe RNA-Seq platform based on the Illumina sequencing system was used to characterize the transcriptomic response during flower blooming by comparing the different transcriptomes at two developmental stages of J. sambac
Jasminum sambac (L.) Ait. (Oleaceae) is a small erect or climbing shrub that is native to Bhutan and India
To understand the molecular basis of flower blooming in J. sambac, the flower budding stage (T1) and flower blooming stage (T2) were used to build two libraries for high-throughput sequencing (Figure 1)
Summary
The RNA-Seq platform based on the Illumina sequencing system was used to characterize the transcriptomic response during flower blooming by comparing the different transcriptomes at two developmental stages of J. sambac.
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