Abstract
Punicalagin showed significant nematotoxic activity against pine wood nematode (PWN), Bursaphelenchus xylophilus, in the authors’ previous research. The authors performed high-throughput transcriptomic sequencing of punicalagin-treated nematodes to generate clues for its nematotoxic mechanism of action. The authors identified 2,575 differentially expressed genes, 1,428 of which were up-regulated and 1,147 down-regulated. Based on a comprehensive functional in silico analysis, the authors speculate that PWN may respond to the stimulus of punicalagin through phagosome, endocytosis, peroxisome and MAPK signaling pathways. In addition, punicalagin could greatly affect PWN energy metabolism including oxidative phosphorylation. The genes encoding twitchin and a nematode cuticular collagen could be crucial regulation targets of punicalagin, which might contribute to its nematotoxic activity against PWN.
Highlights
Pine wood nematode (PWN), Bursaphelenchus xylophilus, is a plant-feeding nematode parasitizing dozens of pine species and is the cause of pine wilt disease
We identified gene regulation patterns correlated with a response to the nematoxin punicalagin
Other enriched pathways included those of cell stress response, endocytosis, peroxisome and MAPK signaling pathways (Fig. A2), which we hypothesize may likewise contribute to the nematodes’ stress response to punicalagin
Summary
Pine wood nematode (PWN), Bursaphelenchus xylophilus, is a plant-feeding nematode parasitizing dozens of pine species and is the cause of pine wilt disease. Transcriptomic analysis, Punicalagin, Nematotoxic mechanism, Pine wood nematode, Differentially expressed genes. Transcriptome analysis of punicalagin-treated Bursaphelenchus xylophilus: Guo et al.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
