Abstract
Bananas are an important part of the diets of millions of people around the globe. Low P absorption and use efficiency significantly restrict banana yields. To further explore the molecular mechanisms of P regulation in banana plants, we used RNA sequencing-based transcriptomic analysis for banana plants subjected to Pi deficit stress for 60 days. We detected 1900 significantly differentially expressed genes (DEGs) in aboveground plant parts and 7398 DEGs in root parts under low P stress. Gene ontology (GO) classification analysis showed that 156,291 GO terms belonging to molecular functions, 53,114 GO terms belonging to cellular components, and 228,544 GO terms belonging to biological processes were enriched in the aboveground and root components. A number of DEGs involved in energy metabolism-related processes, signal transduction, control of rhizosphere P activation, and Pi mobilization were found, which were confirmed by quantitative reverse-transcription Polymerase Chain Reaction (qRT-PCR) analysis. At the transcriptomic level, we detected 13 DEGs from different organs and with different functions in the time-course response to phosphorus deficiency stress. These DEGs may include some key genes that regulate the phosphorus network, increasing our understanding of the molecular mechanism of Pi homeostasis in banana. These findings will also help develop biotechnologies to create a variant of banana with more effective Pi absorption and utilization.
Highlights
Phosphorus is one of the essential macronutrients required for normal plant growth and development
Withered spots were dramatically increased in older leaves, and the whole plant was shorter compared with WT plants after 60 days (Figure 1A)
The expression of an auxin efflux gene (Ma01_g09070) was down-regulated. These results suggest that an increase in the auxin content of banana roots may promote the growth of the plant root and the absorption of phosphorus, which provides better resistance to P starvation stress
Summary
Phosphorus is one of the essential macronutrients required for normal plant growth and development. Low P availability is a global problem restricting yield. The weathering of minerals changes the solubility of P; as the relative abundance of Fe and Al increases, the solubility of P becomes controlled by Fe or Al phosphates [1]. Pi deficiency in the soil limits P absorption by the plant. In order to keep up with the growing global population, the demand for food and energy has led to excessive consumption of Pi fertilizer.
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