Year-end Sale % OFF 
Abstract
Tuberous roots of Aconitum heterophyllum constitute storage organ for secondary metabolites, however, molecular components contributing to their formation are not known. The transcriptomes of A. heterophyllum were analyzed to identify possible genes associated with tuberous root development by taking clues from genes implicated in other plant species. Out of 18 genes, eight genes encoding GDP-mannose pyrophosphorylase (GMPase), SHAGGY, Expansin, RING-box protein 1 (RBX1), SRF receptor kinase (SRF), β-amylase, ADP-glucose pyrophosphorylase (AGPase) and Auxin responsive factor 2 (ARF2) showed higher transcript abundance in roots (13–171 folds) compared to shoots. Comparative expression analysis of those genes between tuberous root developmental stages showed 11–97 folds increase in transcripts in fully developed roots compared to young rootlets, thereby implying their association in biosynthesis, accumulation and storage of primary metabolites towards root biomass. Cluster analysis revealed a positive correlation with the gene expression data for different stages of tuberous root formation in A. heterophyllum. The outcome of this study can be useful in genetic improvement of A. heterophyllum for root biomass yield.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0466-y) contains supplementary material, which is available to authorized users.
Highlights
Aconitum heterophyllum Wall ex Royle (Ranunculaceae), popularly known as Atis, is a biennial herb native to NorthWestern and Eastern Himalayas of India
The transcriptomes of A. heterophyllum were analyzed to identify possible genes associated with tuberous root development by taking clues from genes implicated in other plant species
On the basis of literature survey (Table 1), genes belonging to various physicochemical processes required for tuberous root growth and biomass enhancement were mined in the transcriptomes of A. heterophyllum on the basis of high sequence similarity with known genes (C40 % and E-value threshold of 1e-5)
Summary
Aconitum heterophyllum Wall ex Royle (Ranunculaceae), popularly known as Atis, is a biennial herb native to NorthWestern and Eastern Himalayas of India. Several studies on morphogenesis have been carried out to understand tuberous root development in various plant species such as Ipomoea batatas and Manihot esculenta (Indira and Kurian 1977; Wang et al 2005). Starch biosynthesis genes like AGPase and b-amylase have shown changes in expression pattern during various tuberous root developmental stages in I. batatas (Wang et al 2006). Next-generation sequencing (NGS) transcriptomes analysis of A. heterophyllum root and shoot tissues identified plausible candidate genes responsible for tuberous root formation and development through in silico expression profiling (Pal et al 2015), which have been further assessed for their association with growth and developmental mechanism of tuberous roots. Genes pertaining to primary metabolism in plants, such as starch production, photosynthesis, hormone metabolism and transcription factors were studied to associate their role in storage organ development and root biomass production in A. heterophyllum
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
