Abstract
Inner hair cells (IHCs) and outer hair cells (OHCs) are the two anatomically and functionally distinct types of mechanosensitive receptor cells in the mammalian cochlea. The molecular mechanisms defining their morphological and functional specializations are largely unclear. As a first step to uncover the underlying mechanisms, we examined the transcriptomes of IHCs and OHCs isolated from adult CBA/J mouse cochleae. One thousand IHCs and OHCs were separately collected using the suction pipette technique. RNA sequencing of IHCs and OHCs was performed and their transcriptomes were analyzed. The results were validated by comparing some IHC and OHC preferentially expressed genes between present study and published microarray-based data as well as by real-time qPCR. Antibody-based immunocytochemistry was used to validate preferential expression of SLC7A14 and DNM3 in IHCs and OHCs. These data are expected to serve as a highly valuable resource for unraveling the molecular mechanisms underlying different biological properties of IHCs and OHCs as well as to provide a road map for future characterization of genes expressed in IHCs and OHCs.
Highlights
Background & SummaryHair cells are the sensory receptors of both the auditory system and the vestibular system in the ears of all vertebrates
Much is known about how inner hair cells (IHCs) and outer hair cells (OHCs) function in hearing, we have limited knowledge of molecular mechanisms, i.e., gene expression and regulation, that underlie their distinct morphological and functional specializations
We describe transcriptome-wide profiling of IHCs and OHCs obtained from one-month-old CBA/J mouse cochleae to provide a comprehensive view of the gene expression in IHCs and OHCs
Summary
Hair cells are the sensory receptors of both the auditory system and the vestibular system in the ears of all vertebrates. Transcriptomes of adult IHCs and OHCs from microarray technique[9], as well as neonatal hair cells from RNA-seq[11,12,13,14] were presented along with transcriptomes from the current study. Our dataset is expected to serve as a highly valuable resource for unraveling the molecular mechanisms underlying different biological properties of IHCs and OHCs. The dataset will provide a road map for future characterization of genes expressed in these two types of hair cells and for assisting the auditory research community in exploring the functions of deafness-related genes
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have