Transcriptome-Based Analysis of Dof Family Transcription Factors and Their Responses to Abiotic Stress in Tea Plant (Camellia sinensis).

Hui Li,Jing Zhuang,Zhi-Wei Liu,Yong-Xin Wang,Wei Huang

doi:10.1155/2016/5614142

Abstract

Tea plant (Camellia sinensis (L.) O. Kuntze) is affected by abiotic stress during its growth and development. DNA-binding with one finger (Dof) transcription factors (TFs) play important roles in abiotic stress tolerance of plants. In this study, a total of 29 putative Dof TFs were identified based on transcriptome of tea plant, and the conserved domains and common motifs of these CsDof TFs were predicted and analyzed. The 29 CsDof proteins were divided into 7 groups (A, B1, B2, C1, C2.1, C2.2, and D2), and the interaction networks of Dof proteins in C. sinensis were established according to the data in Arabidopsis. Gene expression was analyzed in “Yingshuang” and “Huangjinya” under four experimental stresses by qRT-PCR. CsDof genes were expressed differentially and related to different abiotic stress conditions. In total, our results might suggest that there is a potential relationship between CsDof factors and tea plant stress resistance.

Highlights

Transcription factors (TFs), known as trans-acting factors, are proteins that recognize and bind specific DNA sequences
A total of 45 amino acid sequences of DNA-binding with one finger (Dof) TFs in Arabidopsis were downloaded from PlnTFDB (Table S3)
The phylogenetic tree was constructed with 29 CsDof TFs in tea plant and 45 AtDof TFs in Arabidopsis

Summary

Introduction

Transcription factors (TFs), known as trans-acting factors, are proteins that recognize and bind specific DNA sequences. These TFs can participate in various biological processes to activate or repress plant metabolic [1], and act as regulators in the process of stem elongation and seed development [2]. Dof TFs regulate the expression of genes involved in plant development and defense processes, such as seed maturation and germination [10,11,12], plant defense mechanisms [13], photoperiodic flowering time [14, 15], and secondary metabolism [16]

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Results

Discussion

Conclusion