Abstract
Panax ginseng C. A. Meyer is a kind of renascent herb that belongs to the genus Panax in the family Araliaceae. It is a traditional Chinese precious herbal medicine with a long history of medicinal use. Ginsenoside Rb3 is one of the important active ingredients in ginseng and has important physiological activity in the treatment of many diseases. In this study, we screened and systematically analyzed the candidate genes related to ginsenoside Rb3 synthesis through bioinformatics methods; discussed the functions, expression patterns, and interactions of the genes related to ginsenoside Rb3 synthesis; and finally, selected seven genes, mainly PgRb3, that directly contribute to the synthesis of ginsenoside Rb3. This study provides a reference for revealing the expression rules of ginsenoside Rb3 synthesis-related genes and elucidating the regulatory mechanism of methyl jasmonate, lays a theoretical foundation for the research of ginsenoside Rb3 synthesis, and provides theoretical and technical support for the factory production of ginsenoside monomer saponins.
Highlights
Through a Methyl jasmonate (MeJA) treatment experiment, we found that PgRb3S01, PgRb3S03, and
Screening of Candidate Genes Related to Ginsenoside Rb3 Synthesis
Through correlation analysis of multiple perspectives, we selected seven genes that have a great contribution to the synthesis of ginsenoside Rb3
Summary
Basic Analysis of Ginsenoside Rb3 Synthesis-Related Genes. An NCBI Conserved Domains Search was used to conduct a conservative domain search 2016) to initially determine the function of ginsenoside Rb3 synthesis-related candidate genes, and the NCBI ORF Finder (https://www.ncbi.nlm.nih.gov/orffinder/, accessed on 13 October 2016) was used to find its open reading frame to determine whether the sequence was to be expressed as a functional protein. Screening of Candidate Genes Related to Ginsenoside Rb3 Synthesis. The expression levels of ginsenoside Rb3 synthesis-related gene groups (in the passage called PgRb3 genes) were divided into two groups from low to high and input into. The genes whose gene expression level changed significantly relative to the content of saponins were selected as candidate genes related to ginsenoside Rb3 synthesis for further analysis. GO (Gene Ontology) Annotation for Candidate Genes Related to Ginsenoside Rb3 Synthesis
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