Abstract
The ubiquitin/26S proteasome pathway is a critical protein-degradation pathway in plant growth and development as well as in nearly all biological and abiotic stress processes. Although as a member of the ubiquitin/26S proteasome pathway, the E3 ubiquitin ligase family has been shown to be essential for the selective degradation of downstream target proteins, it has been rarely reported in tea plants (Camellia sinensis). In this study, through database searches and extensive manual deduplication, 335 RING finger family proteins were selected from the Tea Plant Information Archive. These proteins were divided into six categories by the difference of RING finger domain: RING-H2, RING-HCa, RING-HCb, RING-C2, RING-v, and RING-G. Stress-induced differential gene expression analysis showed that 53 proteins in RING finger family can respond to selected exogenous stress. In vitro ubiquitination assays indicated that TEA031033, which was named CsMIEL1, exhibited the activity of E3 ubiquitin ligases. CsMIEL1-overexpressing transgenic Arabidopsis thaliana seedlings were resistant to some exogenous abiotic stresses, such as salt and drought stress but sensitive to exogenous methyl jasmonate treatment. Furthermore, CsMIEL1 reduced the accumulation of anthocyanin in transgenic plants in response to low temperature treatment. The results of this article provide basic date for studying the role of ubiquitin/26S proteasome pathway in tea plants response to stresses.
Highlights
In contrast to Arabidopsis thaliana, among the 477 RING proteins in Arabidopsis thaliana, 41 (8.6%) HCb-type proteins were present, whereas only 3 HCbs were present in the tea plants
Researchers have thoroughly investigated the RING finger family in Arabidopsis; at least 477 RING proteins have been identified (Stone et al, 2005; Yang et al, 2016), and many genes encoding RING domain proteins have been proven to be involved in cold stress (Dong et al, 2006; Suh et al, 2016), heat stress (Lim et al, 2013; Liu et al, 2016), drought stress (Lee et al, 2009; Ryu et al, 2010), salt stress (Qin et al, 2008; Fang et al, 2015), sugar treatment (Huang et al, 2010), and defense responses (Huang et al, 2010; Marino et al, 2019)
RING proteins have been reported in many species, few studies have explored RING finger family proteins in tea plants
Summary
Kuntze], a perennial evergreen woody plant, encounters various abiotic (drought, heat, cold, and salt) and biological stresses (pests, viruses, and herbivore foraging). Under these stresses, plants undergo various degrees of damage. Under high salt stress, an imbalance of Na+/K+ inside and outside the cell restricts plant growth and accumulates excessive reactive oxygen species, which causes osmotic and even oxidative stresses (Munns and Tester, 2008). Plants can tolerate a harsh environment and maintain normal growth, in recent years, more
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