Abstract
Backgrounds Long noncoding RNAs (lncRNAs) play an important role in various biological processes. However, their functions in salt-sensitive hypertension are largely unknown. In this study, the lncRNA-seq technique was employed to compare the expression profiles of lncRNAs and mRNAs in salt-sensitive hypertensive rats. Methods Blood pressure, serum sodium, and urinary creatinine were texted in salt-sensitive and salt-insensitive rats fed with different salt concentrations. High-throughput sequencing was used to detect the expression of lncRNAs and mRNA in the renal medulla of the two groups. Results Blood pressure and urinary sodium/creatinine of high-salt diets of the sensitive group were significantly higher than that in the control group. Serum sodium has no significant difference between the two groups in high-salt diets. NONRATG007131.2 and NONRATG012674.2 were the most different lncRNAs in the high salt-sensitive group. Correlation analysis reveals that Matn1, Serpinb12, Anxa8, and Hspa5 may play an important role in salt-sensitive hypertension. Conclusion This study analyzed the difference in lncRNA and mRNA between salt-sensitive and salt-insensitive rats with different salt diets by high-throughput sequencing. Salt sensitivity and salt concentration were two key factors for the induction of hypertension. We found some potential genes that play an important role in salt-sensitive hypertension.
Highlights
Hypertension is defined as a complex disease determined by both environmental and genetic factors
Previous studies have reported several noncoding RNAs related to salt-sensitive hypertension [18,19,20,21]; we still have some innovations in the present study. is is the first study to analyze the difference in Long noncoding RNAs (lncRNAs) and mRNA between salt-sensitive and salt-insensitive rats with different salt diets by high-throughput sequencing
The mean blood pressure of rats fed with a high-salt diet was significantly higher at 1 and 2 months compared with rats fed with a lowsalt diet (p < 0.05). ese indicated salt sensitivity and salt concentration were two key factors for the induction of hypertension
Summary
Hypertension is defined as a complex disease determined by both environmental and genetic factors. Long noncoding RNAs (lncRNAs) refer to the nonprotein-coding transcripts that are longer than 200 nucleotides, and they are considered to be nonbiologically functional by-products of the transcription process before In recent years, they have been demonstrated to participate in posttranscriptional regulation, cell growth, differentiation, and proliferation of human genes [14, 15]. Wang’s experimental rats may have a shorter feeding period (7 days), and salt concentration is not very high (4%) Their studies only performed RNA sequencing and failed to collect and analyze some observed indicators (such as blood pressure, blood/ urine sodium, and blood/urine creatinine). By sequencing the medulla lncRNAs in rats, we found differentially expressed lncRNAs in two groups of rats and its associated mRNAs and related pathways. is study provides new therapeutic targets for elucidating the mechanisms of salt-sensitive hypertension-induced renal injury
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