Abstract
BackgroundIt is known that long non-coding RNAs (lncRNAs) play an important role in various biological processes, including cell proliferation, differentiation and apoptosis. However, their functions and profiles in lactation cycle of dairy cows are largely unknown. In this study, lncRNA-seq technique was employed to compare the expression profiles of lncRNAs and mRNAs from Chinese Holstein mammary gland in dry and lactation period.ResultTotally 3746 differentially expressed lncRNAs (DELs) and 2890 differentially expressed genes (DEGs) were identified from the dry and lactation mammary glands of Holstein cows. Functional enrichment analysis on target genes of lncRNAs indicated that these genes were involved in lactation-related signaling pathways, including cell cycle, JAK-STAT, cell adhesion, and PI3K-Akt signaling pathways. Additionally, the interaction between lncRNAs and their potential miRNAs was predicted and partly verified. The result indicated that the lactation-associated miR-221 might interact with lncRNAs TCONS_00040268, TCONS_00137654, TCONS_00071659 and TCONS_00000352, which revealed that these lncRNAs might be important regulators for lactation cycle.ConclusionThis study provides a resource for lncRNA research on lactation cycle of bovine mammary gland. Besides, the interaction between lncRNAs and the specific miRNA is revealed. It expands our knowledge about lncRNA and miRNA biology as well as contributes to clarify the regulation of lactation cycle of bovine mammary gland.
Highlights
It is known that long non-coding RNAs play an important role in various biological processes, including cell proliferation, differentiation and apoptosis
This study provides a resource for long non-coding RNAs (lncRNAs) research on lactation cycle of bovine mammary gland
The objective of this study was to screen the lncRNAs associated with lactation by lncRNA-seq analysis in bovine mammary gland, which would provide a new perspective for lncRNAs in lactation biology and lay the foundation for their further function study in milk composition synthesis and secretion
Summary
It is known that long non-coding RNAs (lncRNAs) play an important role in various biological processes, including cell proliferation, differentiation and apoptosis. Their functions and profiles in lactation cycle of dairy cows are largely unknown. As a downstream regulator of PTEN, miR-486 expressed higher during bovine high quality lactation period and could regulate the secretion of β-casein, lactose and triglyceride in BMECs, which indicated that miR-486 was required for the development of cow mammary gland [17]. MiR-221 regulated lipid metabolism in mammary epithelial cells and was expressed differentially at various stages during mammary gland development [19]. MiR-212 and miR-132 were necessary for mice mammary gland development and growth by targeting MMP9 gene, especially for mammary epithelial ducts [20]
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