Abstract
BackgroundRecurrent spontaneous abortion (RSA) is one of the common complication of pregnancy, bringing heavy burden to the patients and their families. The study aimed to explore the lncRNA-miRNA-mRNA network associated with recurrent spontaneous abortion.MethodsBy transcriptome sequencing, we detected differences in lncRNA, miRNA and mRNA expression in villus tissue samples collected from 3 patients with RSA and 3 normal abortion patients. Differentially expressed lncRNAs, miRNAs and genes (DELs, DEMs and DEGs, respectively) were identified, and Geno Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to determine the functions of DELs and DEGs, which were analysed by Fisher’s test. We also observed the regulatory relationships between miRNA-mRNA and lncRNA-miRNA by Cytoscape 3.6.1.ResultsThe results showed that 1008 DELs (523 upregulated and 485 downregulated), 475 DEGs (201 upregulated and 274 downregulated) and 37 DEMs (15 upregulated and 22 downregulated) were identified. And we also constructed a novel lncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 genes (NTNG2, GRIA1 and AQP1).ConclusionslncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 mRNAs (NTNG2, GRIA1 and AQP1) was constructed. The results may provide a basic theory for elucidating the mechanism underlying RSA.
Highlights
Recurrent spontaneous abortion (RSA) is one of the common complication of pregnancy, bringing heavy burden to the patients and their families
The results described above indicated that Long noncoding RNAs (lncRNAs) played a role in RSA
By assessing the influence of USP25 on trophoblasts, Ding et al found that USP25 expression was negatively regulated by miR27a-3p, and this effect contributed to the pathogenesis of RSA by suppressing the migration and invasion of trophoblasts [11]
Summary
Recurrent spontaneous abortion (RSA) is one of the common complication of pregnancy, bringing heavy burden to the patients and their families. The study aimed to explore the lncRNA-miRNA-mRNA network associated with recurrent spontaneous abortion. Gu et al observed [8] that polymorphisms in lncRNA HULC may be related to the susceptibility to RSA in the Southern Chinese population. Xuan et al found that the lncRNA MALAT1 rs619586 G mutation reduced the risk of RSA [9]. Che et al found that lncRNA CCAT2 rs619586 G mutation may have a potential protective effect and reduce the risk of RSA in southern China [10]. LncRNA-miRNA interactions and lncRNA-miRNAmRNA networks have not been reported in RSA
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