Abstract
Background Trichoderma brevicompactum is the Trichoderma species producing simple trichothecenes-trichodermin, a potential antifungal antibiotic and a protein synthesis inhibitor. However, the biosynthetic pathway of trichodermin in Trichoderma is not completely clarified. Therefore, transcriptome and gene expression profiling data for this species are needed as an important resource to better understand the mechanism of the trichodermin biosynthesis and provide a blueprint for further study of T. brevicompactum.ResultsIn this study, de novo assembly of the T. brevicompactum transcriptome using the short-read sequencing technology (Illumina) was performed. In addition, two digital gene expression (DGE) libraries of T. brevicompactum under the trichodermin-producing and trichodermin-nonproducing culture conditions, respectively, were constructed to identify the differences in gene expression. A total of 23,351 unique transcripts with a mean length of 856 bp were obtained by a new Trinity de novo assembler. The variations of the gene expression under different culture conditions were also identified. The expression profiling data revealed that 3,282 unique transcripts had a significantly differential expression under the trichodermin-producing condition, as compared to the trichodermin-nonproducing condition. This study provides a large amount of transcript sequence data that will contribute to the study of the trichodermin biosynthesis in T. brevicompactum. Furthermore, quantitative real-time PCR (qRT-PCR) was found to be useful to confirm the differential expression of the unique transcripts.ConclusionOur study provides considerable gene expression information of T. brevicompactum at the transcriptional level,which will help accelerate the research on the trichodermin biosynthesis. Additionally, we have demonstrated the feasibility of using the Illumina sequencing based DGE system for gene expression profiling, and have shed new light on functional studies of the genes involved in T. brevicompactum biosynthesis.
Highlights
The filamentous fungus Trichoderma brevicompactum was firstly identified and confirmed in 2004 [1]
T. brevicompactum (IBT 9471) had been mistaken for T. harzianum (ATCC 90237) on the basis of considerable shared micromorphology, and later this IBT 9471 strain was reclassified as T. arundinaceum based on the phylogenetic lineage within the morphological species T. brevicompactum and trichothecene production feature [1,3,4]
We found the unigenes homologous to those involved in the trichothecene biosynthesis in Fusarium and T. arundinaceum were up-regulated under the trichodermin-producing condition
Summary
The filamentous fungus Trichoderma brevicompactum was firstly identified and confirmed in 2004 [1]. According to the current study, T. brevicompactum is the Trichoderma species producing simple trichothecenes-trichodermin [2,3,4], a potent antifungal antibiotic and a protein synthesis inhibitor in mammalian cells [5,6]. By using a polyphasic (morphological characters, sequence analysis and substrate assimilation patterns) approach, T. brevicompactum was recognized as a member of Brevicompactum clade, a separate lineage in Trichoderma/Hypocrea. This includes T. brevicompactum and 4 other species 2T. Trichoderma brevicompactum is the Trichoderma species producing simple trichothecenes-trichodermin, a potential antifungal antibiotic and a protein synthesis inhibitor. Transcriptome and gene expression profiling data for this species are needed as an important resource to better understand the mechanism of the trichodermin biosynthesis and provide a blueprint for further study of T. brevicompactum
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
