Abstract
In order to explore the molecular mechanism of regulating flowering in Prunus sibirica , the transcriptome of flower buds at two different stages was sequenced to investigate the candidate genes involved in the flowering. A total of 42.04 Gb clean data were obtained, and more than 92.00% of the clean reads were mapped to the reference genome. A total of 6 850 significantly differentially expressed genes (DEGs) were found, including 2 784 significantly up-regulated and 4 066 significantly down regulated genes. We further found 392 and 346 DEGs were special in sprouting stage and full-bloom stage, respectively. KEGG enrichment analysis showed that the first three DEGs were enriched in plant hormone signal transduction, phenylpropanoid biosynthesis, and starch and sucrose metabolism, respectively. Among these DEGs, 39 genes involved in the flowering regulation pathways were obtained, including 10, 17, 5, 1, and 2 genes in vernalization pathway, photoperiod pathway, autonomous pathway, gibberellin pathway, and temperature pathway, respectively. In addition, four integrators, including SOC1 , FT , and LFY , were also identified. This study will provide the valuable information for studying the candidate genes involved in the flowering and for breeding in P. sibirica .
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