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Abstract
Background Ananas comosus var. bracteatus (Red Pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. Because of its complex genome, it is difficult to understand the molecular mechanisms involved in the growth and development. Thus high-throughput transcriptome sequencing of Ananas comosus var. bracteatus is necessary to generate large quantities of transcript sequences for the purpose of gene discovery and functional genomic studies.ResultsThe Ananas comosus var. bracteatus transcriptome was sequenced by the Illumina paired-end sequencing technology. We obtained a total of 23.5 million high quality sequencing reads, 1,555,808 contigs and 41,052 unigenes. In total 41,052 unigenes of Ananas comosus var. bracteatus, 23,275 unigenes were annotated in the NCBI non-redundant protein database and 23,134 unigenes were annotated in the Swiss-Port database. Out of these, 17,748 and 8,505 unigenes were assigned to gene ontology categories and clusters of orthologous groups, respectively. Functional annotation against Kyoto Encyclopedia of Genes and Genomes Pathway database identified 5,825 unigenes which were mapped to 117 pathways. The assembly predicted many unigenes that were previously unknown. The annotated unigenes were compared against pineapple, rice, maize, Arabidopsis, and sorghum. Unigenes that did not match any of those five sequence datasets are considered to be Ananas comosus var. bracteatus unique. We predicted unigenes encoding enzymes involved in terpenoid and phenylpropanoid biosynthesis.ConclusionThe sequence data provide the most comprehensive transcriptomic resource currently available for Ananas comosus var. bracteatus. To our knowledge; this is the first report on the de novo transcriptome sequencing of the Ananas comosus var. bracteatus. Unigenes obtained in this study, may help improve future gene expression, genetic and genomics studies in Ananas comosus var. bracteatus.
Highlights
Pineapple is a perennial, herbaceous monocot of the family Bromeliaceae, sub family Bromelioideae, in the order Poales
In total 41,052 unigenes of Ananas comosus var. bracteatus, 23,275 unigenes were annotated in the National center for Biotechnology (NCBI) non-redundant protein database and 23,134 unigenes were annotated in the Swiss-Port database
In recent years transcriptome sequencing has become an effective tool to discover the unigenes at a given moment in time
Summary
Herbaceous monocot of the family Bromeliaceae, sub family Bromelioideae, in the order Poales. Pineapple crop was grown for its stem and leaves, which were a source of high quality silk fiber, widely used in paper and clothes industry [2,3]. It is source of bromelain, a proteolytic enzyme complex, used in the meat industry, but due to its health benefits, it is commercially used in pharmaceutical industry, since they contain substances and vitamins that are beneficial for human health [4]. Bracteatus (Red Pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. Highthroughput transcriptome sequencing of Ananas comosus var. Highthroughput transcriptome sequencing of Ananas comosus var. bracteatus is necessary to generate large quantities of transcript sequences for the purpose of gene discovery and functional genomic studies
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