Abstract
Potato is an important food crop and its production is susceptible to drought. Drought stress in crop growth is usually multiple- or long-term. In this study, the drought tolerant potato landrace Jancko Sisu Yari was treated with drought stress, rehydration and re-dehydration, and RNA-seq was applied to analyze the characteristics of gene regulation during these treatments. The results showed that drought-responsive genes mainly involved photosynthesis, signal transduction, lipid metabolism, sugar metabolism, wax synthesis, cell wall regulation, osmotic adjustment. Potato also can be recovered well in the re-emergence of water through gene regulation. The recovery of rehydration mainly related to patatin, lipid metabolism, sugar metabolism, flavonoids metabolism and detoxification besides the reverse expression of the most of drought-responsive genes. The previous drought stress can produce a positive responsive ability to the subsequent drought by drought hardening. Drought hardening was not only reflected in the drought-responsive genes related to the modified structure and cell components, but also in the hardening of gene expression or the “memory” of drought-responsive genes. Abundant genes involved photosynthesis, signal transduction, sugar metabolism, protease and protease inhibitors, flavonoids metabolism, transporters and transcription factors were subject to drought hardening or memorized drought in potato.
Highlights
Potato is the most important food crop after wheat and rice [1]
Transcriptome analysis showed that transcription factors, protein kinases, proteins related to redox regulation, carbohydrate metabolism and osmotic adjustment participate in the tolerance of potato to osmotic stress [8,9,10]
Twenty-three drought-responsive genes were identified by comparing the transcriptomes of drought-tolerant and drought-sensitive potato varieties under water stress, and seven homologous genes were identified by homozygous mutants of Arabidopsis thaliana, six of them including carbohydrate transporter, mitogen-activated protein kinase kinase kinase 15 (MAPKKK15), serine-like carboxypeptidase 19 (SCPL19), armadillo/beta-catenin-like repeat-containing protein, high-affinity nitrate transporter 2.7 and nonspecific lipid transfer protein 2 can improve the drought resistance of Arabidopsis [11]
Summary
Potato is the most important food crop after wheat and rice [1]. It is more sensitive to water deficit than other crops, and if it suffers from drought during the critical growth period, its yield and quality will decrease significantly [2,3]. Twenty-three drought-responsive genes were identified by comparing the transcriptomes of drought-tolerant and drought-sensitive potato varieties under water stress, and seven homologous genes were identified by homozygous mutants of Arabidopsis thaliana, six of them including carbohydrate transporter, mitogen-activated protein kinase kinase kinase 15 (MAPKKK15), serine-like carboxypeptidase 19 (SCPL19), armadillo/beta-catenin-like repeat-containing protein, high-affinity nitrate transporter 2.7 and nonspecific lipid transfer protein 2 (nsLPT) can improve the drought resistance of Arabidopsis [11]. These results suggest the complexity of the molecular mechanism of potato drought-resistance. Transcriptome analysis of JSY under continuous treatments of dehydration/rehydration/re-dehydration was performed for profiling the response to drought, rehydration, and re-dehydration stress and for elaborating the molecular mechanism of drought resistance in potato more comprehensively
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