Abstract
Tomato yellow leaf curl virus (TYLCV) is a serious pathogen transmitted by the whitefly (Bemisia tabaci). Due to the quick spread of the virus, which is assisted by its vector, tomato yield and quality have suffered a crushing blow. Resistance to TYLCV has been intensively investigated in transmission, yet the mechanism of anti-TYLCV remains elusive. Herein, we conducted transcriptome profiling with a TYLCV-resistant cultivar (CLN2777A) and a susceptible line (Moneymaker) to identify the potential mechanism of resistance to TYLCV. Compared to the susceptible line, CLN2777A maintained a lower level of lipid peroxidation (LPO) after TYLCV infection. Through RNA-seq, over 1000 differentially expressed genes related to the metabolic process, cellular process, response to stimulus, biological regulation, and signaling were identified, indicating that the defense response was activated after the virus attack. Further analysis showed that TYLCV infection could induce the expression of the genes involved in salicylic and jasmonic acid biosynthesis and the signal transduction of phytohormones, which illustrated that phytohormones were essential for tomatoes to defend against TYLCV. These findings provide greater insight into the effective source of resistance for TYLCV control, indicating a potential molecular tool for the design of TYLCV-resistant tomatoes.
Highlights
Received: 7 December 2021Tomato yellow leaf curl virus (TYLCV), belonging to the genus Begomovirus, is a devastating pathogen and can lead to great crop loss, especially in tropical and subtropical regions with high heat and humidity [1]
48 h of infection of TYLCV, the contents of Jasmonic acid (JA) and ABA were obviously affected. These in the first 48 h of infection of TYLCV, the contents of JA and ABA were obviously results indicate that tomato plants of T2 probably regulate the biosynthesis of salicylic acid (SA) to defend affected. These results indicate that tomatoes probably regulate the biosynthesis of SA to against pathogen attack, making sure that plants produce more zeatin biosynthesis (ZT) and indole-3-acetic acid (IAA) for growth
Our results showed that both SA biosynthesis and signal genes were dramatically induced by TYLCV at the time of infection (Figure 5A), which illustrates that SA acts as the principal defender
Summary
Received: 7 December 2021Tomato yellow leaf curl virus (TYLCV), belonging to the genus Begomovirus (familyGeminiviridae), is a devastating pathogen and can lead to great crop loss, especially in tropical and subtropical regions with high heat and humidity [1]. Tomato yellow leaf curl virus (TYLCV), belonging to the genus Begomovirus The migration of infected whitefly (Bemisia tabaci) is considered the main route of transmission. In addition to vegetables such as tomatoes, peppers, and cucumbers, TYLCV can infect various crops, including tobacco, cassava, and cotton, and can cause great damage to their production [6]. As the main host of TYLCV, the typical symptom in tomatoes induced by the virus is arrested development with upward curling of the leaves and chlorosis, which results in a reduced yield and poor quality [7,8]. It is hard to completely eliminate TYLCV or control an outbreak of it as its vector, Bemisia tabaci, has a strong reproduction capability and migration rate
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
