Transcriptome Profiling Provides Molecular Insights into Auxin-Induced Adventitious Root Formation in Sugarcane (Saccharum spp. Interspecific Hybrids) Microshoots

Aomei Li,Weizhong He,Limin Liu,Prakash Lakshmanan,Hongwei Tan,Hongjian Liu,Dongliang Huang,Zhongliang Chen,Junxian Liu

doi:10.3390/plants9080931

Abstract

Adventitious root (AR) formation was enhanced following the treatment of sugarcane microshoots with indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) combined, suggesting that auxin is a positive regulator of sugarcane microshoot AR formation. The transcriptome profile identified 1737 and 1268 differentially expressed genes (DEGs) in the basal tissues (5 mm) of sugarcane microshoots treated with IBA+NAA compared to nontreated control on the 3rd and 7th days post-auxin or water treatment (days post-treatment—dpt), respectively. To understand the molecular changes, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. This analysis showed that DEGs associated with the pathways were associated with plant hormone signaling, flavonoid and phenylpropanoid biosyntheses, cell cycle, and cell wall modification, and transcription factors could be involved in sugarcane microshoot AR formation. Furthermore, qRT–PCR analysis was used to validate the expression patterns of nine genes associated with root formation and growth, and the results were consistent with the RNA-seq results. Finally, a hypothetical hormonal regulatory working model of sugarcane microshoot AR formation is proposed. Our results provide valuable insights into the molecular processes associated with auxin-induced AR formation in sugarcane.

Highlights

IntroductionSugarcane (Saccharum spp. interspecific hybrids), a major industrial crop, contributes approximately
Sugarcane (Saccharum spp. interspecific hybrids), a major industrial crop, contributes approximately80% of global sugar production with an annual value of USD150 billion [1]
indole-3-butyric acid (IBA)+naphthalene acetic acid (NAA) mixture or water were carefully removed from the soil, and the number and length of roots were measured

Summary

Introduction

Sugarcane (Saccharum spp. interspecific hybrids), a major industrial crop, contributes approximately. 80% of global sugar production with an annual value of USD150 billion [1]. Complex genome due to its high and variable chromosome number and polyploidy [2]. Sugarcane is propagated vegetatively for commercial crop production [3]. Nodal cuttings of sugarcane stem, called billets, or the entire stem were used for planting commercial crops. The traditional method of propagating sugarcane planting material in the field has increasingly been replaced by micropropagation. Micropropagation has the advantages of rapid production and scale-up potential

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Results

Discussion

Conclusion