Abstract
Bean (Phaseolus vulgaris) anthracnose caused by the hemi-biotrophic pathogen Colletotrichum lindemuthianum is a major factor limiting production worldwide. Although sources of resistance have been identified and characterized, the early molecular events in the host-pathogen interface have not been investigated. In the current study, we conducted a comprehensive transcriptome analysis using Illumina sequencing of two near isogenic lines (NILs) differing for the presence of the Co-1 gene on chromosome Pv01 during a time course following infection with race 73 of C. lindemuthianum. From this, we identified 3,250 significantly differentially expressed genes (DEGs) within and between the NILs over the time course of infection. During the biotrophic phase the majority of DEGs were up regulated in the susceptible NIL, whereas more DEGs were up-regulated in the resistant NIL during the necrotrophic phase. Various defense related genes, such as those encoding PR proteins, peroxidases, lipoxygenases were up regulated in the resistant NIL. Conversely, genes encoding sugar transporters were up-regulated in the susceptible NIL during the later stages of infection. Additionally, numerous transcription factors (TFs) and candidate genes within the vicinity of the Co-1 locus were differentially expressed, suggesting a global reprogramming of gene expression in and around the Co-1 locus. Through this analysis, we reduced the previous number of candidate genes reported at the Co-1 locus from eight to three. These results suggest the dynamic nature of P. vulgaris–C. lindemuthianum interaction at the transcriptomic level and reflect the role of both pathogen and effector triggered immunity on changes in plant gene expression.
Highlights
MethodsA near isogenic (NIL) pair of bean genotypes was used in the present study
Anthracnose caused by Colletotrichum lindemuthianum (Sacc. & Magnus)-Briosi & Cavara, is a major pathogen of common bean (Phaseolus vulgaris L.) worldwide, but is PLOS ONE | DOI:10.1371/journal.pone.0165823 November 9, 2016Transcriptome of Bean- Anthracnose Pathosystem
These results confirm the hemibiotrophic nature of the fungus and that the biotrophic phase is active until 72 h, when the fungus switches to the necrotrophic phase after the colonized tissue is dead
Summary
A near isogenic (NIL) pair of bean genotypes was used in the present study. The NILs were developed from among three heterogeneous F4:6 recombinant inbred lines (RILs) [50] extracted from a 96-member RIL population derived from the cross of Jaguar and Puebla 152 black bean cultivars. Jaguar possess the Co-1 gene that provides resistance to race 73 of C. lindemuthianum whereas Puebla 152 lacks the resistance allele [23, 51]. The final NIL pair selected (10T-9576) was similar in all phenotypic characters except in response to inoculation with race 73 of C. lindemuthianum. The NIL (T-9576R) with resistance to race 73 of C. lindemuthianum possessed the Co-1 gene on chromosome Pv01, whereas the susceptible NIL (T-9576S) possessed the susceptible co-1 allele. The phenotypic reaction of the NIL pair was confirmed by inoculation prior to the initiation of the study
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