Abstract
In crustaceans, muscle growth and development is complicated, and to date substantial knowledge gaps exist. In this study, the claw muscle, hepatopancreas and nervous tissue of the mud crab (Scylla paramamosain) were collected at three fattening stages for sequence by the Illumina sequencing. A total of 127.87 Gb clean data with no less than 3.94 Gb generated for each sample and the cycleQ30 percentages were more than 86.13% for all samples. De Bruijn assembly of these clean data produced 94,853 unigenes, thereinto, 50,059 unigenes were found in claw muscle. A total of 121 differentially expressed genes (DEGs) were revealed in claw muscle from the three fattening stages with a Padj value < 0.01, including 63 genes with annotation. Functional annotation and enrichment analysis showed that the DEGs clusters represented the predominant gene catalog with roles in biochemical processes (glycolysis, phosphorylation and regulation of transcription), molecular function (ATP binding, 6-phosphofructokinase activity, and sequence-specific DNA binding) and cellular component (6-phosphofructokinase complex, plasma membrane, and integral component of membrane). qRT-PCR was employed to further validate certain DEGs. Single nucleotide polymorphism (SNP) analysis obtained 159,322, 125,963 and 166,279 potential SNPs from the muscle transcriptome at stage B, stage C and stage D, respectively. In addition, there were sixteen neuropeptide transcripts being predicted in the claw muscle. The present study provides a comprehensive transcriptome of claw muscle of S. paramamosain during fattening, providing a basis for screening the functional genes that may affect muscle growth of S. paramamosain.
Highlights
The mud crab Scylla paramamosain belonging to genus Scylla, is widely distributed along inshore region in the southeast China coasts and other Asian countries [1]
This paper reported the results of next-generation sequencing (Illumina sequencing) which pay attention to claw muscle growth during mud crab fattening
Crabs at three different fattening stages were sampled, according to the molt cycle of the swimming crab Portunus trituberculatus [11], they attribute to stage B, stage C and stage D
Summary
The mud crab Scylla paramamosain belonging to genus Scylla, is widely distributed along inshore region in the southeast China coasts and other Asian countries [1]. Due to the short growth cycle and the large market demand, the mud crab has high commercial value in farming [2]. The aquaculture of this species has emerged more than 100 years in China and more than 30 years in other Asian countries [1, 3]. The lower yields own to many factors, such as growth, nutrition, diseases, and administration patterns [4]. Research into mechanism underlying the growth and development is of obvious importance.
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