Transcriptome profiling of anthocyanin biosynthesis in the peel of \u2018Granny Smith\u2019 apples (Malus domestica) after bag removal

Changqing Ma,Yazhou Yang,Bowen Liang,Jiuying Yan,Zhengyang Zhao,Ying Wang,Bo Chang,Li Liu

doi:10.1186/s12864-019-5730-1

Changqing Ma, Yazhou Yang + Show 6 more

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https://doi.org/10.1186/s12864-019-5730-1

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Journal: BMC Genomics	Publication Date: May 9, 2019
Citations: 31	License type: open-access

Affiliation: North West Agriculture and Forestry University

Abstract

BackgroundBagging is commonly used to enhance red pigmentation and thereby improve fruit quality of apples (Malus domestica). The green-skinned apple cultivar ‘Granny Smith’ develops red pigmentation after bagging removal, but the signal transduction pathways mediating light-induced anthocyanin accumulation in apple peel are yet to be defined. The aim of this study was to identify the mechanisms underpinning red pigmentation in ‘Granny Smith’ after bag removal based on transcriptome sequencing.ResultsThe anthocyanin content in apple peel increased considerably after bag removal, while only trace amounts of anthocyanins were present in the peel of unbagged and bagged fruits. RNA sequencing identified 18,152 differentially expressed genes (DEGs) among unbagged, bagged, and bag-removed fruits at 0, 4, and 10 days after bag removal. The DEGs were implicated in light signal perception and transduction, plant hormone signal transduction, and antioxidant systems. Weighted gene co-expression network analysis of DEGs generated a module of 23 genes highly correlated with anthocyanin content. The deletion of − 2026 to − 1870 bp and − 1062 to − 964 bp regions of the MdMYB1 (LOC103444202) promoter induced a significant decrease in glucuronidase activity and anthocyanin accumulation in apple peel.ConclusionsBagging treatment can induce red pigmentation in ‘Granny Smith’ via altering the expression patterns of genes involved in crucial signal transduction and biochemical metabolic pathways. The − 2026 to − 1870 bp and − 1062 to − 964 bp regions of the MdMYB1 promoter are essential for MdMYB1-mediated regulation of anthocyanin accumulation in the ‘Granny Smith’ apple cultivar. The findings presented here provide insight into the mechanisms of coloration in the peel of ‘Granny Smith’ and other non-red apple cultivars.

Highlights

Bagging is commonly used to enhance red pigmentation and thereby improve fruit quality of apples (Malus domestica)
The red pigmentation of fruits removed from bags [160 days after full bloom (DAFB)] was associated with an elevation (0 to 0.39 mg g− 1) in the anthocyanin content of fruit peel samples from 0 to 10 days after bag removal (DABR), while only trace amounts of anthocyanins were observed in unbagged (~ 0.01 mg g− 1) and bagged (0 mg g− 1) fruits (Fig. 1b)
These findings indicate that the red pigmentation can be mainly attributed to the accumulation of anthocyanins in the peel of ‘Granny Smith’ apples

Summary

Introduction

Bagging is commonly used to enhance red pigmentation and thereby improve fruit quality of apples (Malus domestica). The structural genes associated with anthocyanin biosynthesis in apple have been characterized, including phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), 4-coumarate coenzyme A ligase (4CL), chalcone isomerase (CHI), dihydroflavonol 4-reductase (DFR), flavanone 3-hydroxylase (F3H), anthocyanidin synthase/leucoanthocyanidin dioxygenase (ANS), and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) [7, 8]. All these structural genes are regulated by a conserved MYB-bHLH-WD40/ WDR (MBW) regulatory complex [9].

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