Transcriptome profiling of <italic>glossy1</italic> mutant with glossy glume in common wheat (<italic>Triticum aestivum </italic>L.)

Abstract

<p id="C3">In order to clarify the molecular mechanism of the significant change in glume wax content, transcriptome profiling was performed on glossy glume mutant <italic>glossy1 </italic>and its wild-type Jimai 22. The results showed that a total of 12,230 differentially expressed genes were screened in the <italic>glossy1</italic> mutant, among which 5811 genes were up-regulated and 6419 were down-regulated. GO functional enrichment revealed that the differentially expressed genes were mainly enriched in the wax synthesis and transport pathway, specifically distributed in acyltransferase activity, lipid binding, hydrolase activity. The findings suggested that these pathways were closely related to the glume-wax-deficient trait in wheat. We also detected the relative expression levels of some genes involved in the wax metabolic pathway by RT-qPCR, and the results were consistent with the transcriptome data. This study not only provides data support for the further study of the molecular mechanism of wax metabolism and gene regulation network but also lays a theoretical foundation for the breeding of stress-resistant variety in wheat.