Abstract
Chinese sucker (Myxocyprinus asiaticus), a unique species of Catostomidae in Asia and China's second-class protected animal species, has important value both in research and economy field. Moreover, Chinese sucker are susceptible to infection by Aeromonas hydrophila. However, until now the information related to the immune system against A. hydrophila of Chinese sucker is unclear. Therefore, to understand the molecular basis underlying the host immune response to A. hydrophila infection, Illumina HiSeq™ 2000 was used to analyse the spleen transcriptome of infected Chinese sucker for the first time. De nova assembly of paired-end reads yielded 693,660 unigenes. Comparative analysis of the expression profile between bacteria challenge fish and control fish identified a total of 1390 differentially expressed genes (padj<0.05 and |log2FoldChange| > 1), including 907 up-regulated genes and 483 down-regulated genes. A significant enrichment analysis of these DEGs in spleen revealed major immune-related pathways, including Toll-like receptor, T cell receptor, complement and coagulation cascades, MAPK and NF-kB. The selected DEGs were validated using qPCR. Moreover, the expression level of nineteen immune-related genes were detected at 4, 12 and 24 h post infection. The results showed that a various types of molecules were involved in the antibacterial immune response, including pattern recognition receptors (TLR2, TLR4, TLR6 and PGRP5), interleukins (IL1B, IL6, IL10, IL11 and IL12), chemokines (CXCL1, CXCL2 and CXCL8), activity regulation molecules of immune cells (CD68, CD200, CD247, CSF3 and CSF3R), and complement components (C6, C7). Altogether, the results obtained on immune-related genes may allow for a better understanding of immunity in Chinese sucker to A. hydrophila, carrying out detailed functional analysis of these genes and developing strategies for efficient immune protection against infections in Chinese sucker, which will be great significance for healthy breeding and resource conservation of Chinese sucker.
Published Version
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