Abstract
Radish is a common crop and vulnerable to high temperature. Pretreatment with spermidine could effectively elevate the resistance of radish to high temperature. Up to date, the underlying mechanisms remain unknown. In the present study, radish seedlings were pretreated with spermidine and then exposed to high temperature. The leaves were collected for transcriptome sequencing and the results were compared with samples only exposed to high temperature and samples consistently cultured at normal temperature. The differentially expressed genes were identified and validated using real-time quantitative PCR. Finally, the results revealed that pretreatment with spermidine significantly up-regulated the transcription level of anti-oxidation enzymes, heat-shock proteins, and proteins involved in the photosynthetic process, in comparison with treatments with high temperature and room temperature, suggesting that pretreatment with spermidine increased heat tolerance in radish probably through minimizing the harms caused by peroxidation, accelerating the corrections or degradation of misfolded proteins, and promoting photosynthesis. These findings preliminarily uncovered the molecular mechanisms underlying the spermidine-promoted heat resistance in radish.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.