Abstract
Adaptation of S. cerevisiae to toxic concentrations of manganese provides a physiological model of heavy metal homeostasis. Transcriptome analysis of adapted yeast cells reveals upregulation of cell wall and plasma membrane proteins including membrane transporters. The gene expression in adapted cells differs from that of cells under short-term toxic metal stress. Among the most significantly upregulated genes are PMA2, encoding an ortholog of Pma1 H+-ATPase of the plasma membrane, and YBR056W-A, encoding a putative membrane protein Mnc1 that belongs to the CYSTM family and presumably chelates manganese at the cell surface. We demonstrate that these genes are essential for the adaptation to toxic manganese concentration and propose an extended scheme of manganese detoxification in yeast.
Highlights
Introduction a Skryabin Institute ofBiochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr
The cells of S. cerevisiae adapted to 2.5 mM of manganese after a prolonged lag-phase are characterized by specific changes in morphology.[18]
The GO-enrichment analysis performed with YeastMine[30] revealed downregulation of various cellular biosynthesis processes, which agrees with the observed decrease in the growth rate
Summary
E-mail: alla@ibpm.pushchino.ru b Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, 127051, Russia c A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia d Laboratory of Extreme Biology, Institute of Fundamental Biology and Medicine, Kazan Federal University, Kazan, 420012, Russia e Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia f Group of Protein Biosynthesis Regulation, Institute of Protein Research, Institutskaya 4, Pushchino, 142290, Russia g Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina 3, Moscow, GSP-1, 119991, Russia h Moscow Institute of Physics and Technology (State University), Institutskiy per. 9, Dolgoprudny, Moscow Region, 141700, Russia i Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova 32, Moscow, GSP-1, 119991, Russia. E-mail: ivan.kulakovskiy@gmail.com † Electronic supplementary information (ESI) available: Supplementary figures (S1–S5) and supplementary tables (S1 and S2).
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