Transcriptome profile analysis of cell proliferation molecular processes during multicellular trichome formation induced by tomato Wov gene in tobacco.

Changxian Yang,Shenghua Gao,Cheng Xiong,Zhibiao Ye,Hanxia Li,Yanna Gao,Gang Yu,Jiang Chang

doi:10.1186/s12864-015-2099-7

Abstract

BackgroundTrichomes, developing from the epidermis of nearly all terrestrial plants, provide good structural resistance against insect herbivores and an excellent model for studying the molecular mechanisms underlying cell fate determination. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation molecular processes during multicellular trichome formation in Asterids.ResultsIn this study, we identified two point mutations in a novel allele (Wov) at Wo locus. Ectopic expression of Wov in tobacco and potato induces much more trichome formation than wild type. To gain new insights into the underlying mechanisms during the processes of these trichomes formation, we compared the gene expression profiles between Wov transgenic and wild-type tobacco by RNA-seq analysis. A total of 544 co-DEGs were detected between transgenic and wild-type tobacco. Functional assignments of the co-DEGs indicated that 33 reliable pathways are altered in transgenic tobacco plants. The most noticeable pathways are fatty acid metabolism, amino acid biosynthesis and metabolism, and plant hormone signal transduction. Results suggest that these enhanced processes are critical for the cell proliferation during multicellular trichome formation in transgenic plants. In addition, the transcriptional levels of homologues of trichome regulators in Rosids were not significantly changed, whereas homologues of genes (Wo and SlCycB2) in Asterids were significantly upregulated in Wov transgenic tobacco plants.ConclusionsThis study presents a global picture of the gene expression changes induced by Wov- gene in tobacco. And the results provided us new insight into the molecular processes controlling multicellular formation in tobacco. Furthermore, we inferred that trichomes in solanaceous species might share a common network.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-2099-7) contains supplementary material, which is available to authorized users.

Highlights

Trichomes, developing from the epidermis of most terrestrial plants, provide good structural resistance against insect herbivores and an excellent model for studying the molecular mechanisms underlying cell fate determination
We amplified and sequenced the genomic sequences of this locus from LA3560 and determined two nucleotide substitutions in its 3′ portion of the coding region by using wo as reference (Accession number: JF518780) (Fig. 1b). We determined that these two point mutations resulted in one amino acid replacement (Ile-692 to Arg, Asp-695 to Tyr) separately (Fig. 1c)
Ectopic expression of tomato Wov in tobacco and potato induces multicellular trichome formation To investigate whether the formation of trichomes in tobacco and tomato is controlled by the common

Summary

Introduction

Trichomes, developing from the epidermis of most terrestrial plants, provide good structural resistance against insect herbivores and an excellent model for studying the molecular mechanisms underlying cell fate determination. Ectopic expression of Wov in tobacco and potato induces much more trichome formation than wild type. To gain new insights into the underlying mechanisms during the processes of these trichomes formation, we compared the gene expression profiles between Wov transgenic and wild-type tobacco by RNA-seq analysis. The most noticeable pathways are fatty acid metabolism, amino acid biosynthesis and metabolism, and plant hormone signal transduction Results suggest that these enhanced processes are critical for the cell proliferation during multicellular trichome formation in transgenic plants. The identification of these genes, including TRANSPARENT TESTA GLABRA1 (TTG1), GLABRA3 (GL3), and ENHANCER OF GLABRA3 (EGL3), has elucidated the molecular mechanism of trichome formation [6,7,8] These genes induce trichome initiation in the form of a MYB/bHLH/WD repeat complex [9]. GaHOX1 and GaHOX3 with high sequence similarities to GL2 could activate trichome initiation when expressed in Arabidopsis [19]

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Conclusion