Abstract
BackgroundCyclocybe aegerita (syn. Agrocybe aegerita) is a commercially cultivated mushroom. Its archetypal agaric morphology and its ability to undergo its whole life cycle under laboratory conditions makes this fungus a well-suited model for studying fruiting body (basidiome, basidiocarp) development. To elucidate the so far barely understood biosynthesis of fungal volatiles, alterations in the transcriptome during different developmental stages of C. aegerita were analyzed and combined with changes in the volatile profile during its different fruiting stages.ResultsA transcriptomic study at seven points in time during fruiting body development of C. aegerita with seven mycelial and five fruiting body stages was conducted. Differential gene expression was observed for genes involved in fungal fruiting body formation showing interesting transcriptional patterns and correlations of these fruiting-related genes with the developmental stages. Combining transcriptome and volatilome data, enzymes putatively involved in the biosynthesis of C8 oxylipins in C. aegerita including lipoxygenases (LOXs), dioxygenases (DOXs), hydroperoxide lyases (HPLs), alcohol dehydrogenases (ADHs) and ene-reductases could be identified. Furthermore, we were able to localize the mycelium as the main source for sesquiterpenes predominant during sporulation in the headspace of C. aegerita cultures. In contrast, changes in the C8 profile detected in late stages of development are probably due to the activity of enzymes located in the fruiting bodies.ConclusionsIn this study, the combination of volatilome and transcriptome data of C. aegerita revealed interesting candidates both for functional genetics-based analysis of fruiting-related genes and for prospective enzyme characterization studies to further elucidate the so far barely understood biosynthesis of fungal C8 oxylipins.
Highlights
IntroductionSeveral studies revealed that the volatile profile of mushrooms differs depending on the developmental stage [41,42,43,44,45,46,47,48,49]
In S. commune for example, the deletion of the transcription factor HOM2 was associated with an enhanced growth of vegetative mycelium unable to develop Fruiting bodies (FBs) whereas the deletion of HOM1 and GAT1 resulted in the formation of more but smaller FBs with an unusual morphology compared to the wild type [10, 12]
Differential gene expression during fruiting body development To identify changes in the transcriptome during mushroom tissue formation and FB maturation in C. aegerita, RNA sequencing of different developmental stages of mycelium and FBs was conducted (Additional file 1)
Summary
Several studies revealed that the volatile profile of mushrooms differs depending on the developmental stage [41,42,43,44,45,46,47,48,49] In this context, the function of VOCs as ‘infochemicals’ is of special interest since VOCs have proven to influence the behavior of invertebrates and play probably an important role in the fungal life cycle by inter alia repelling fungal predators or attracting insects for the purpose of spore dispersal (reviewed in [50, 51]). The changes observed in volatilomes of fungi are probably due to the adaption of the organisms to the altering requirements during different developmental stages
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
