Abstract
The response and adaptation to drought remains poorly understood for Paulownia australis. To investigate this issue, transcriptome profiling of four P. australis accessions (two diploid and the other two autotetraploid) under water stress condition were studied using Illumina Genome Analyzer IIx analysis. The current study aimed to identify genes of P. australis metabolism pathways that might be involved in this plant’s response to water deficit. Potted seedlings were subjected to well-watered conditions and drought stress, respectively. More than 290 million raw transcript reads were assembled into 111,660 unigenes, with a mean length of 1013 bp. Clusters of orthologous groups, gene ontology and the Kyoto Encyclopedia of Genes and Genomes annotations analyses were performed on the unigenes. Many differentially expressed genes and several metabolic pathways were identified. Quantitative real-time polymerase chain reaction was used to verify the expression patterns of 14 genes. Our study identified altered gene expression in P. australis induced by drought stress and provided a comprehensive map of drought-responsive genes and pathways in this species. To our knowledge, this is the first publicly available global transcriptome study of P. australis. This study provides a valuable genetic resource for this species.
Highlights
Adverse environmental influences affect the survival, reproductive biology, stability and productivity of plants [1]
The changing trends of leaf physiological and biochemical indexes were consistent with the aggravation of drought stress (Figure 1)
The water and chlorophyll contents of the leaves decreased during drought stress
Summary
Adverse environmental influences affect the survival, reproductive biology, stability and productivity of plants [1]. High-throughput sequencing techniques have become more affordable; analysis of drought-induced genome-wide transcription by RNA-seq, for example, is currently feasible This method has been used to efficiently mine responsive genes under drought stress in model plants and agronomically important crop species [5]. The plant response to drought is a polygenic trait that involves the activation of a cascade of genes, whose functions range from water deficiency perception to stress signal transmission. These genes can be categorized into two major groups based on their putative biological function in the process of drought response. The genes and pathways related to the drought response represent putative candidates for manipulation to improve the adaptation to drought of this plant or even other species
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