Abstract
Enrofloxacin is the most commonly used antibiotic to control diseases in aquatic animals caused by A. hydrophila. This study conducted de novo transcriptome sequencing and compared the global transcriptomes of enrofloxacin-resistant and enrofloxacin-susceptible strains. We got a total of 4,714 unigenes were assembled. Of these, 4,122 were annotated. A total of 3,280 unigenes were assigned to GO, 3,388 unigenes were classified into Cluster of Orthologous Groups of proteins (COG) using BLAST and BLAST2GO software, and 2,568 were mapped onto pathways using the Kyoto Encyclopedia of Gene and Genomes Pathway database. Furthermore, 218 unigenes were deemed to be DEGs. After enrofloxacin treatment, 135 genes were upregulated and 83 genes were downregulated. The GO terms biological process (126 genes) and metabolic process (136 genes) were the most enriched, and the terms for protein folding, response to stress, and SOS response were also significantly enriched.This study identified enrofloxacin treatment affects multiple biological functions of A. hydrophila. Enrofloxacin resistance in A. hydrophila is closely related to the reduction of intracellular drug accumulation caused by ABC transporters and increased expression of topoisomerase IV.
Highlights
Following the decline in the capture fishing industry and diminishing wild fish stocks, the aquaculture industry has become an important source of food fish [1]
We examined the transcriptome of A. hydrophila using the Illumina sequencing platform and explored the molecular mechanism of enrofloxacin resistance in A. hydrophilia
Compared with the reference genome, the total mapped rates of reads were 94.19% and 93.29% in the A. hydrophilia transcriptome of the enrofloxacin-susceptible (ATCC 7966) and enrofloxacin-resistant (ATCC 7966QR) strains, respectively, indicating that the quality of sequencing data met the demand for follow-up studies
Summary
Following the decline in the capture fishing industry and diminishing wild fish stocks, the aquaculture industry has become an important source of food fish [1]. The gram-negative bacterium Aeromonas hydrophila is one of the major causative agents of disease and can cause serious damage in many animals [2], especially fish [3,4] as well as humans [5]. A. hydrophila, which is a representative of the Aeromonadaceae family, is an emerging aquatic pathogen that is distributed in a wide variety of aquatic systems [6,7]. It primarily inhabits freshwater and the intestines of freshwater animals. Farmers use a wide range of antibiotics or chemicals to control A. hydrophila
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