Abstract

Tissue culture environment liberate cells from ordinary laws of multi-cellular organisms. This liberation enables cells several behaviors, such as growth, dedifferentiation, acquisition of pluripotency, immortalization and reprogramming. Each phenomenon is relating to each other and hardly to determine. Recently, dedifferentiation of animal cell was quantified as increasing liberality which is information entropy of transcriptome. The increasing liberality induced by tissue culture may reappear in plant cells too. Here we corroborated it. Measuring liberality during reprogramming of plant cells suggested that reprogramming is a combined phenomenon of dedifferentiation and re-differentiation.

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