Abstract
Wild barley eibi1 mutant with HvABCG31 gene mutation has low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. To better understand how such a mutant plant survives, we performed a genome-wide gene expression analysis. The leaf transcriptomes between the near-isogenic lines eibi1 and the wild type were compared using the 22-k Barley1 Affymetrix microarray. We found that the pleiotropic effect of the single gene HvABCG31 mutation was linked to the co-regulation of metabolic processes and stress-related system. The cuticle development involved cytochrome P450 family members and fatty acid metabolism pathways were significantly up-regulated by the HvABCG31 mutation, which might be anticipated to reduce the levels of cutin monomers or wax and display conspicuous cuticle defects. The candidate genes for responses to stress were induced by eibi1 mutant through activating the jasmonate pathway. The down-regulation of co-expressed enzyme genes responsible for DNA methylation and histone deacetylation also suggested that HvABCG31 mutation may affect the epigenetic regulation for barley development. Comparison of transcriptomic profiling of barley under biotic and abiotic stresses revealed that the functions of HvABCG31 gene to high-water loss rate might be different from other osmotic stresses of gene mutations in barley. The transcriptional profiling of the HvABCG31 mutation provided candidate genes for further investigation of the physiological and developmental changes caused by the mutant.
Highlights
IntroductionThe drought-hypersensitive mutant eibi was obtained from a wild barley
The drought-hypersensitive mutant eibi1 was obtained from a wild barley (Hordeum spontaneumKoch) accession in Israel [1]
0.5-fold in eibi1 compared to the wild type (Figure 1)
Summary
The drought-hypersensitive mutant eibi was obtained from a wild barley The excessive water loss of the eibi mutant plant was related to a recessive mutation localized in a pericentromeric region of chromosome 3H [2]. A candidate gene for eibi, based on high resolution genetic mapping was reported [3]. The difficulty of barley transformation could not allow the functional analysis of the candidate gene with respect to its phenotypes. Availability of microarray platforms representing a large proportion of barley genes has enabled the application of transcriptomic analysis to several known mutations in barley including biotic and abiotic stress-related genes [7,8,9,10,11]. To better understand how eibi1/HvABCG31 mutant displayed the defective physiological and growth phenotypes, we performed a genome-wide gene expression analysis by using Affymetrix Barley. The comparative analysis of eibi to other barley transcriptome components under various stress response signals was revealed
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