Abstract

The transportation is a crucial phase in beef cattle industry, and the annual losses caused by beef cattle transport stress are substantial. Several studies have described the effect of long distance transportation stress on animal health, such as disorder in nervous, endocrine, immune, and metabolic system. However, molecular mechanisms underlying short distance transportation stress is still poorly understood. Present study aims to investigate the effect of short distance transportation by measuring the hematological indices and transcriptomic analysis. In this study, a total 10 Qinchuan cattle were used to compare the molecular characteristics of blood before and after transportation. We have found that a stress-related marker “white blood cell count (WBC)” increased significantly after transportation. The decrease in triglyceride (TG), cholestenone (CHO), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) showed that energy expenditure was increased after transportation, but not enough to activate fatty decomposition. Intriguingly, the decrease of malondialdehyde (MDA) showed that cattle were more resilience to oxidative stress. The RNA-seq showed that 1,092 differentially expressed genes (DEGs) were found (329 up-regulated and 763 down-regulated) between group before and group after. The GO and KEGG enrichment showed that the metabolic pathway and B cell function related pathways were enriched. Furthermore, median absolute deviation (MAD) top 5,000 genes were used to construct a co-expression network by weighted correlation network analysis (WGCNA), and 11 independent modules were identified. Combing with protein-protein interaction (PPI) analysis, the verification of quantitative real-time PCR (qPCR) and the correlation of B cell function, structural maintenance of chromosomes 3 (SMC3), jun proto-oncogene (JUN), and C-X-C motif chemokine ligand 10 (CXCL10) were suggested as potential molecular markers in identification of short distance transportation. Collectively, the blood RNA-seq analysis and WGCNA indicated that the disorder of B cell differentiation, proliferation, survival, and apoptosis were the potential molecular mechanism in short distance transportation stress. In conclusion, our results provide the novel insight about potential biomarkers for short distance transportation stress, which may serve as for diagnosing and preventing this condition in beef industry.

Highlights

  • Cut meat has become a major product in the meat market worldwide

  • The white blood cell count (WBC), MON#, Gran#, Gran%, MCHC, platelet number (PLT) and PCT% were higher after transportation compare to before transportation

  • The TG, CHO, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and MDA contents were lower after transportation compare before transportation, whereas GLU and Superoxide dismutase (SOD) levels did not differ significantly between the two stages (Figure 2)

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Summary

Introduction

Cut meat has become a major product in the meat market worldwide. Livestock is bought and sold more frequently in the market economy and transportation is an integral part of the livestock industry. The key factors that can affect the welfare of animals and the economic benefits of this industry during transportation include a higher susceptibility to disease and increased disease spread (Schwartzkopf-Genswein et al, 2012; Deng et al, 2017). Transportation stress syndrome (TSS) often appears in beef cattle after ground transportation, and can affect many tissues, including the nervous, endocrine, immune, and energy supply systems (Van Engen and Coetzee, 2018). To avoid the losses caused by TSS and better safeguard animal welfare, it is essential to provide a better condition and immune enhancing supplements for cattle during transportation.

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