Transcriptome Characterization and Identification of Molecular Markers (SNP, SSR, and Indels) in the Medicinal Plant Sarcandra glabra spp.

Yanqin Xu,Fei Ge,Wenzhen Huang,Shuyun Tian,Renqing Li,Xiaofang Huang,Yin Zhou,Fengqin Li,Biaolin Hu

doi:10.1155/2021/9990910

Abstract

Sarcandra glabra has significant metabolically active bioingredients of pharmaceutical importance. The deficiency of molecular markers for S. glabra is a hindrance in molecular breeding for genetic improvement. In this study, 57.756 million pair-end reads were generated by transcriptome sequencing in S. glabra (Thunb.) Nakai and its subspecies S. glabra ssp. brachystachys. A total of 141,954 unigenes with 646.63 bp average length were assembled. A total of 25,620 simple sequence repeats, 726,476 single nucleotide polymorphisms, and 42,939 insertions and deletions were identified, and the associated unigenes and differentially expressed genes were characterized. This work enhanced the molecular marker resources and will facilitate molecular breeding and gene mining in S. glabra spp.

Highlights

Sarcandra glabra (Chloranthaceae family) is an evergreen, smooth shrub, and grows up to one-meter height
The genetic improvement of S. glabra as a pharmacologically important plant is required, which could be accelerated by breeding strategies employing molecular markers
A molecular plant breeding program is always a fast and cost-effective tool in genomic improvement but it demands a rich molecular marker resource that is deficient for S. glabra

Summary

Introduction

Sarcandra glabra (Chloranthaceae family) is an evergreen, smooth shrub, and grows up to one-meter height It is originated in tropical climate of south Asia. It especially grows in China, Japan, Philippines, Vietnam, Korea, Cambodia, Malaysia, India, Sri Lanka, and other areas including areas of North, Central, and South America and some areas of the Pacific [1]. It has been known for its medicinal values in the treatment of joint and cancer problems [2]. Owing to its significant chemical, rheological, and pharmacological importance [1, 2], it is important to improve of S. glabra varieties with wider acceptance and enriched with bioactive components

Methods

Results

Conclusion