Transcriptome-Based Identification and Molecular Evolution of the Cytochrome P450 Genes and Expression Profiling under Dimethoate Treatment in Amur Stickleback (Pungitius sinensis).

Jun Cao,Xiuzhu Cheng

doi:10.3390/ani9110873

Abstract

Simple SummaryCytochrome P450s (CYPs) are a family of membrane-bound monooxygenase proteins. In this study, 58 CYP genes were identified in Amur stickleback (Pungitius sinensis). Motif distribution, recombination, and selection were performed to investigate their evolutionary history. In addition, expression profiles of CYPs were examined following dimethoate treatment. The results will provide a useful reference for further functional analyses.Cytochrome P450s (CYPs) are a family of membrane-bound mono-oxygenase proteins, which are involved in cell metabolism and detoxification of various xenobiotic substances. In this study, we identified 58 putative CYP genes in Amur stickleback (Pungitius sinensis) based on the transcriptome sequencing. Conserved motif distribution suggested their functional relevance within each group. Some present recombination events have accelerated the evolution of this gene family. Moreover, a few positive selection sites were identified, which may have accelerated the functional divergence of this family of proteins. Expression patterns of these CYP genes were investigated and indicated that most were affected by dimethoate treatment, suggesting that CYPs were involved in the detoxication of dimethoate. This study will provide a foundation for the further functional investigation of CYP genes in fishes.

Highlights

Cytochrome P450s (CYPs) are a family of membrane-bound hemoproteins that catalyze a variety of mono-oxygenase reactions, which play key roles in detoxification and cellular metabolism of xenobiotics [1]
In order to evaluate the evolutionary relationship among CYP family genes in this fish, phylogenetic analysis was carried out based on a NJ (Neighbor-Joining) method
The CYP1 family belongs to Group I, which is involved in the metabolism and activation of carcinogens in fish [54]

Summary

Introduction

Cytochrome P450s (CYPs) are a family of membrane-bound hemoproteins that catalyze a variety of mono-oxygenase reactions, which play key roles in detoxification and cellular metabolism of xenobiotics [1]. CYPs are divided into different families and subfamilies based on their special function and species existence. Different families are represented by Arabic numerals, while different subfamilies are represented by capital letters from A to Q [4]. CYP1B2 refers to CYP family 1, subfamily B and protein 2. There are more than 13,000 CYP genes representing more than 400 families among various species [5] The number of CYP genes varies greatly among different organisms [6,7,8,9].

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