Abstract
Background: The causal agent of pine wilt disease is the pine wood nematode (PWN) (Bursaphelenchus xylophilus), whose ability to adapt different ecological niches is a crucial determinant of their invasion to colder regions. To discover the molecular mechanism of low temperature response mechanism, we attempted to study the molecular response patterns under low temperature from B. xylophilus with a comprehensive RNA sequencing analysis and validated the differentially expressed genes (DEGs) with quantitative real-time polymerase chain reaction (qRT-PCR). Bioinformatic software was utilized to isolate and identify the low-temperature-related BxGPCR genes. Transcript abundance of six low-temperature-related BxGPCR genes and function of one of the BxGPCR genes are studied by qRT-PCR and RNA interference. Results: The results showed that we detected 432 DEGs through RNA sequencing between low-temperature-treated and ambient-temperature-treated groups nematodes. The transcript level of 6 low-temperature-related BxGPCR genes increased at low temperature. And, the survival rates of BxGPCR17454 silenced B. xylophilus revealed a significant decrease at low temperature. Conclusion: in conclusion, this transcriptome-based study revealed a crucial role of BxGPCR17454 in low temperature response process of pine wood nematode. These discoveries would assist the development of management and methods for efficient control of this devastating pine tree pest.
Highlights
As one of the most dangerous plant pests in the world, pine wood nematode (Bursaphelenchus xylophilus, PWN) causes devastating pine wilt diseases to the pine trees in Asia, Europe, and North America [1]
Through the analysis of differentially expressed genes (DEGs) sets, we identified transcriptome changes in B. xylophilus in response to low temperature and validated the transcriptome result with Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
The DEG analysis revealed that many gene groups including heat shock proteins, cytochrome P450s and G protein coupled receptors (GPCRs), among others, may potentially have played a crucial role in low temperature response process of B. xylophilus
Summary
As one of the most dangerous plant pests in the world, pine wood nematode (Bursaphelenchus xylophilus, PWN) causes devastating pine wilt diseases to the pine trees in Asia, Europe, and North America [1]. The causal agent of pine wilt disease is the pine wood nematode (PWN) (Bursaphelenchus xylophilus), whose ability to adapt different ecological niches is a crucial determinant of their invasion to colder regions. Conclusion: in conclusion, this transcriptome-based study revealed a crucial role of BxGPCR17454 in low temperature response process of pine wood nematode. These discoveries would assist the development of management and methods for efficient control of this devastating pine tree pest
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