Abstract
BackgroundAlthough selenium (Se) plays important roles in scavenging free radicals, alleviating oxidative stresses, and strengthening immune system, the knowledge about Se responses in bread wheat is still limited. In order to clarify the molecular mechanism of Se responses in bread wheat, 2-week-old wheat seedlings of cultivar ‘Jimai22’ treated with 10 μM disodium selenate (Na2SeO4) for 0, 3, and 24 h were collected and analyzed by transcriptional sequencing and tandem mass tag-based (TMT) quantitative proteomics.ResultsAt least 11,656 proteins and 133,911 genes were identified, and proteins including ATP sulfurylase (APS), cysteine synthase (CS), SeCys lyase, sulfate transporters, glutathione S-transferase (GSTs), glutathione peroxidase (GSH-Px), glutaredoxins (GRXs), superoxide dismutases (SODs), catalases (CATs), heat shock proteins (HSPs), UDP-glycose flavonoid glycosyltransferases (UFGTs), sucrose-6-phosphate hydrolases (Suc-6-PHs), archaeal phosphoglucose isomerases (APGIs), malate synthases (MSs), and endo-1,4-beta-xylanase (Xyn) in Se accumulation, ROS scavenging, secondary metabolism, and carbohydrate metabolism were significantly differently expressed.ConclusionsThis is the first complementary analyses of the transcriptome and proteome related with selenium responses in bread wheat. Our work enhances the understanding about the molecular mechanism of selenium responses in bread wheat.
Highlights
Selenium (Se) plays important roles in scavenging free radicals, alleviating oxidative stresses, and strengthening immune system, the knowledge about Se responses in bread wheat is still limited
Different se accumulation in divergent wheat cultivars In order to detect the differences of Se accumulation in divergent wheat cultivars, 5 main wheat cultivars in Huang and Huai River Wheat Zone of China including Jimai22 (JM22), Luyuan502 (LY502), Qingmai6 (QM6), WO4, and Jinan17 (JN17) were selected, and the Se contents in their dried seeds were detected
The results indicated that JM22 had highest Se contents in these 5 wheat cultivars, and the Se contents in JM22 was about 4 fold of others (Supplementary material 1)
Summary
Selenium (Se) plays important roles in scavenging free radicals, alleviating oxidative stresses, and strengthening immune system, the knowledge about Se responses in bread wheat is still limited. Selenium (Se) is a prosthetic group of many enzymes including glutathione peroxidase (GSH-Px), thioredoxin reductase (TrxR), and iodothyronine-deiodinase, so it plays important roles in scavenging free radicals, alleviating oxidative stresses, and strengthening immune systems [1]. Se may be absorbed in the form of selenate (SeO42−), selenite (SeO32−), and organic Se. Selenate is the main form of Se in alkaline soils, whereas selenite is more predominantly presented in acid and neutral soils than other forms of Se [4]. Due to similar size and charge between selenate and sulfate (SO42−), the selenate is taken in plants through sulfate transporters and reduced to selenite [5]. The selenite is taken in plants by phosphate (PO43−)
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